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Gm08398

Manufactured by Coriell Institute for Medical Research
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Sourced in United Kingdom

GM08398 is a cell line obtained from a human individual. It is a lymphoblastoid cell line, which means it was derived from a B lymphocyte that was transformed by Epstein-Barr virus. This cell line is available from the Coriell Institute for Medical Research for research purposes.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Gm05659, by Coriell Institute for Medical Research (2 mentions) Gm02036, by Coriell Institute for Medical Research (2 mentions) Gm05756, by Coriell Institute for Medical Research (2 mentions) Essential 8 medium, by Thermo Fisher Scientific (2 mentions) Matrigel, by Corning (2 mentions) Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions) Pen strep glutamine, by Thermo Fisher Scientific (1 mentions) Fbs fetal bovine serum, by Gemini Bio (1 mentions) Pen strep, by Thermo Fisher Scientific (1 mentions) Ag11513, by Coriell Institute for Medical Research (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Corning (1 mentions)

5 protocols using gm08398

1

Fibroblast Culture and Single-Cell Sorting

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Two WT control fibroblast cell lines (GM08398 and GM00409) and three BS patient fibroblast cell lines (GM02520, GM02932 and GM02548) were purchased from Coriell Institute for Medical Research (see Supplementary Material, Table S1 for details). The cells were maintained in Dulbecco’s Modified Eagle Medium (Gibco, Life Technologies) supplemented with 10% fetal bovine serum (Superior; Sigma-Aldrich), 100 U/ml penicillin, and 100 μg/ml streptomycin in a 37°C incubator with 5% CO2. On the day of sc sorting, cells were harvested with 0.05% trypsin-EDTA (Gibco, Life Technologies) and diluted in 1× DPBS before further steps.
Gönenc I.I., Wolff A., Schmidt J., Zibat A., Müller C., Cyganek L., Argyriou L., Räschle M., Yigit G, & Wollnik B. (2022). Single-cell transcription profiles in Bloom syndrome patients link BLM deficiency with altered condensin complex expression signatures. Human Molecular Genetics, 31(13), 2185-2193.
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2

Culturing Primary Dermal Fibroblasts for Research

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Primary dermal fibroblast cells were cultured from proband skin biopsies. Unaffected sex-matched primary dermal fibroblast cell lines GM23972 (Control A) and GM08398 (Control B) (Coriell Institute for Medical Research, Camden, NJ) were used as controls. Control A fibroblasts were submitted to a research study as a normal control with clinically normal brain MRI scans, neurological exam, and neuropsychological testing. Control B fibroblasts were sampled from the inguinal area and described as an “apparently healthy collection.” Fibroblasts were cultured at 37 °C with 5% CO2 in standard growth media: 90% DMEM – Dulbecco’s Modified Eagle Medium (11,995, Gibco/Thermofisher), 10% FBS – Fetal Bovine Serum (100–500, GeminiBio), and 1× PenStrep Glutamine (10,378,016, Gibco/Thermofisher). Fibroblasts were cultured until 70–80% confluency.
Johnstone T., Wang J., Ross D., Balanda N., Huang Y., Godfrey R., Groden C., Barton B.R., Gahl W., Toro C, & Malicdan M.C. (2020). Biallelic variants in two complex I genes cause abnormal splicing defects in probands with mild Leigh syndrome. Molecular genetics and metabolism, 131(1-2), 98-106.
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3

Culturing Human Dermal Fibroblasts

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Human primary dermal fibroblast cell lines were obtained from The Progeria Research Foundation (PRF) Cell and Tissue Bank (HGPS: HGADFN167 and healthy adult: HGADFN168). Dermal fibroblasts (HGPS: AG11513, healthy adult: AG03257, and healthy child GM08398) were purchased from Coriell Institute (Camden, NJ). Cells were grown in DMEM (Gibco) supplemented with 15% FBS (Corning), 1% Pen-strep (Gibco), and 1% L-glutamine (Gibco). Cells were passaged at a density of 80%.
San Martin R., Das P., Sanders J.T., Hill A.M, & McCord R.P. (2022). Transcriptional profiling of Hutchinson-Gilford Progeria syndrome fibroblasts reveals deficits in mesenchymal stem cell commitment to differentiation related to early events in endochondral ossification. eLife, 11, e81290.
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4

Generating Human iPSCs from Skin Fibroblasts

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Skin fibroblasts derived from healthy donors (GM02036 from 11-year female, GM05659 from 1-year male, GM05756 from 2-month male, and GM08398 from 8-year male) were obtained from Coriell Institute for Medical Research (Camden, NJ). Fibroblasts were cultured in DMEM (Life Technologies 11195–073) supplemented with 10% fetal bovine serum (FBS), 100 units/ml penicillin and 100 μg/ml streptomycin (Life technologies 10378–016) in a humidified incubator with 5% CO2 and 20% O2 at 37 °C. Cells were routinely trypsinized (0.05% trypsin/EDTA) and passaged until reprogramming. Human iPSCs were maintained on Matrigel (Corning, #354230) in the chemically-defined Essential 8 medium (Life Technologies, #A1517001) and passaged using 0.5 mM EDTA/DPBS, as described previously (Beers et al., 2012 (link)) (Table 1).
Xu X., Pradhan M., Xu M., Cheng Y.S., Beers J., Linask K.L., Lin Y., Zheng W, & Zou J. (2020). Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals. Stem cell research, 49, 102011.
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5

Generating Human iPSCs from Skin Fibroblasts

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Skin fibroblasts derived from healthy donors (GM02036 from 11-year female, GM05659 from 1-year male, GM05756 from 2-month male, and GM08398 from 8-year male) were obtained from Coriell Institute for Medical Research (Camden, NJ). Fibroblasts were cultured in DMEM (Life Technologies 11195–073) supplemented with 10% fetal bovine serum (FBS), 100 units/ml penicillin and 100 μg/ml streptomycin (Life technologies 10378–016) in a humidified incubator with 5% CO2 and 20% O2 at 37 °C. Cells were routinely trypsinized (0.05% trypsin/EDTA) and passaged until reprogramming. Human iPSCs were maintained on Matrigel (Corning, #354230) in the chemically-defined Essential 8 medium (Life Technologies, #A1517001) and passaged using 0.5 mM EDTA/DPBS, as described previously (Beers et al., 2012 (link)) (Table 1).
Xu X., Pradhan M., Xu M., Cheng Y.S., Beers J., Linask K.L., Lin Y., Zheng W, & Zou J. (2020). Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals. Stem cell research, 49, 102011.
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