Monoclonal anti cdc42 antibodies

Manufactured by BD

Monoclonal anti-Cdc42 antibodies are laboratory reagents used for the detection and study of the Cdc42 protein. Cdc42 is a member of the Rho family of small GTPases that regulate various cellular processes. These antibodies can be used in techniques such as Western blotting, immunoprecipitation, and immunocytochemistry to identify and quantify the Cdc42 protein in biological samples.

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Lab products found in correlation

Monoclonal rabbit, by Cell Signaling Technology (2 mentions) Rhodamine phalloidin, by Thermo Fisher Scientific (2 mentions) Anti gfp monoclonal antibody, by BD (2 mentions) Monoclonal rat, by Roche (2 mentions) Anti ha, by Abcam (1 mentions)

Close spelling variants of this product

Anti-Cdc42 Cdc42

2 protocols using monoclonal anti cdc42 antibodies

Integrin Activation and Signaling Assay

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Rhodamine–phalloidin (for actin staining) was from Invitrogen (Carlsbad, CA); anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH), anti-HA, phospho-paxillin (Y118), monoclonal anti-Cdc42 antibodies, anti-GFP monoclonal antibody, active 9EG7 and total Hmβ1-1 CD29 β1-integrin, and monoclonal paxillin antibodies were from BD Biosciences (Palo Alto, CA); rat immunoglobulin G (IgG) a,κ and hamster IgG isotype controls were from Biolegend (San Diego, CA). Monoclonal rat from Roche Molecular Biochemicals (Indianapolis, IN) or monoclonal rabbit (from Cell Signaling Technology, Beverly, MA) anti-HA antibody was used for cell staining; antibodies against mouse FAK, phospho-FAK (Y397), vinculin, and α-tubulin were from Abcam (Cambridge, MA). Mouse monoclonal pan-arrestin F4C1 antibody recognizing epitope DGVVLVD in the N-domain was a generous gift of L. A. Donoso (Wills Eye Hospital, Philadelphia, PA). Arrestins were detected with arrestin-2-specific (1:6000; Mundell et al., 1999 (link)) or arrestin-3-specific (1:700; Orsini and Benovic, 1998 (link)) affinity-purified rabbit polyclonal antibodies. Total integrin antibody M-106 for Western blot was from Santa Cruz Biotechnology (Santa Cruz, CA).

Cleghorn W.M., Branch K.M., Kook S., Arnette C., Bulus N., Zent R., Kaverina I., Gurevich E.V., Weaver A.M, & Gurevich V.V. (2015). Arrestins regulate cell spreading and motility via focal adhesion dynamics. Molecular Biology of the Cell, 26(4), 622-635.

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Immunofluorescence Staining Reagents and Antibodies

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Rhodamine-phalloidin (for actin staining) was from Invitrogen (Carlsbad, CA); anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), anti-HA, monoclonal anti-Cdc42 antibodies, anti-GFP monoclonal antibody, and monoclonal paxillin antibodies were from BD Biosciences (Palo Alto, CA); monoclonal rat from Roche Molecular Biochemicals (Indianapolis, IN) or monoclonal rabbit (from Cell Signaling Technology) anti-HA antibodies were used for cell staining; antibodies against mouse FAK were from Abcam (Cambridge, MA). Pan-arrestin mouse monoclonal F4C1 antibody [32 (link)] was a gift of Dr. L. A. Donoso. Monoclonal RhoA antibody was a part of the Rho activation pulldown kit (Cytoskeleton, Denver, CO). Anti-Rac1 mouse monoclonal antibody was from Cytoskeleton (Denver, CO).

Cleghorn W.M., Bulus N., Kook S., Gurevich V.V., Zent R, & Gurevich E.V. (2017). Non-visual arrestins regulate the focal adhesion formation via small GTPases RhoA and Rac1 independently of GPCRs. Cellular signalling, 42, 259-269.

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