Anxa8 sirna

RPE cells were plated in full media and allowed to attach. SiRNA transfection was carried out at 80% cell confluence using a pool of AnxA8 siRNA (Life Technologies) with the following sequences: human AnxA8 siRNA-1: 5′-CAG CCU UUC GGU CUU CUA UTT-3′, human AnxA8 siRNA-2: GCG UGA UGG GAC CCU GAU ATT-3′, human AnxA8 siRNA-3: 5′-GCC CUU AUG UAC CCG CCA UTT-3′^{54 (link)}. Cells were transfected using Interferin (Polyplus transfection) for 48 h, and each AnxA8 siRNA was added at 150 nM. Allstars siRNA (Qiagen) was used as a control. Cells were washed and incubated with full media for another 48 h. Phase images were taken, and cells were processed for real-time PCR and immunofluorescence analysis.