Isoflow sheath fluid
The IsoFlow Sheath Fluid is a specialized laboratory equipment product designed to provide a consistent and controlled flow of sheath fluid for flow cytometry applications. It is a crucial component in maintaining the optimal performance and accuracy of flow cytometry instruments.
Lab products found in correlation
11 protocols using isoflow sheath fluid
Quantification of NIS and PENDRIN Proteins
Profiling T Cell Subsets and Tfh
To determine the Tfh, at least 1 × 106 PBMC were washed by Fascflow (BD Biosciences, New Jersey, US) and stained with CD3 BV510 (Biolegend, California, US), CD4 BV421 (Biolegend, California, US), CXCR5 Alexa Fluor 647 (BD Biosciences, New Jersey, US), and PD1 APC-Cy7 (Biolegend, California, US) for 30 min at room temperature in the dark.
Mutagenesis and Screening of C. cellulolyticum DPEase
Apoptosis Assay in HCEnC-21T Cells
Phenotypic Characterization of Stem Cells
MN-Induced Apoptosis in NQO1 Knockout Cells
Flow Cytometric Analysis of Immune Complexes
The sheath fluid for the cytometer was IsoFlow™ Sheath Fluid from Beckman Coulter, Part Number 8448010, an isotonic fluid at a pH 7.35-7.65, with Sodium Phosphate Dibasic, Sodium Fluoride, Diethylene Glycol Phenyl Ether, and 2-Phenoxyethanol.
A microparticle gate was established in agreement with the published method for characterization of circulating cell-derived microparticles by preliminary standardization experiments using a blend of size-calibrated 0.3, 0.5, 0.9, and 3 μm fluorescent beads, Megamix®, Biocytex, Marseille, France [27 (link), 28 (link)]. To enlarge the scale of fluorescent beads, in some experiments, Trucount beads (Beckton Dickinson) of 10 μm were added.
A gate for DNA-anti-DNA immune complexes for each series of experiments was built around DNA-anti-DNA immune complexes observed in the aliquot of the pool of 15 SLE serum samples incubated with calf thymus DNA. See below for more details and for compensations.
Erythrocyte Membrane Particles Analysis
Automated Algal Cell Size Measurement
Simultaneous Apoptosis and GD2 Staining
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