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2 protocols using biochanin a

1

Flavonoid Compound Characterization

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The following flavonoids were used in this study: biochanin A (>98%, Tokyo Chemical Industry, B4098), (+)-catechin hydrate (98%, Tokyo Chemical Industry, C0705), chrysin (98%, Alfa Aesar, L14178), cyanidin chloride (98%, Fujifilm Wako Chemicals, 030-21961), daidzein (DAI; ≥98%, Nagara Science, NH010102), desmethylglycitein (DMG; >95%, Tokyo Chemical Industry, T3473), S-equal (≥97%, Merck, SML2147), fisetin (>96%, Tokyo Chemical Industry, T0121), genistein (>98%, Tokyo Chemical Industry, G0272), 2′-hydroxyflavanone (>98%, Tokyo Chemical Industry, H1024), kaempferol (≥98%, Cayman Chemical Company, 11852), luteolin (95%, Fujifilm Wako Chemicals, 127-06241), myricetin (>97%, Tokyo Chemical Industry, M2131), naringenin (>98%, Alomone Labs, N-110), petunidin (≥98%, Cayman Chemical Company, 19755), quercetin (96.5%, Fujifilm Wako Chemicals, 512-58344), tamarixetin (>99%, Extrasynthese, 1140S), 5,3′,4′-trihydroxyflavone (>85%, Toronto Research Chemicals, T896685), and 7,3′,4′-trihydroxyflavone (>85%, Toronto Research Chemicals, T896780).
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2

Purification and Characterization of YjiC

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Genistein, daidzein, biochanin A and formononetin were purchased from Tokyo Chemical Industry (Japan). UDP-α-D-glucose was purchased from Sigma-Aldrich (USA). Genistein 7-O-β-D-glucoside (genistin) was available in our laboratory. All other chemicals and reagents were of the highest chemical grade available. The high resolution mass spectrometry spectra were obtained in positive ion mode on ACQUITY (UPLC, Waters Corp., USA) coupled with SYNAPT G2-S (Waters Corp.). The details of the methodology for the cloning, expression, and purification of YjiC are described in our previous report (Pandey et al., 2013a (link)).
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