Cxcr3 fitc

Manufactured by Thermo Fisher Scientific

Sourced in United States

CXCR3-FITC is a fluorescently-labeled antibody that binds to the CXCR3 receptor, which is expressed on the surface of certain immune cells. It is designed for use in flow cytometry applications to identify and quantify CXCR3-positive cells within a sample.

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Lab products found in correlation

Cd8 pe, by Thermo Fisher Scientific (1 mentions) Mouse anti ki67, by Thermo Fisher Scientific (1 mentions) Chitosan, by Solarbio (1 mentions) Folic acid, by Merck Group (1 mentions) Fixation buffer, by BioLegend (1 mentions) Cd62l fitc, by BD (1 mentions) Cd4 apc cy7, by BD (1 mentions) Cd3 efluor450, by Thermo Fisher Scientific (1 mentions) Cd44 percp cy5, by BD (1 mentions) Foxp3 pe, by BD (1 mentions) Cd8 apc, by BD (1 mentions) Foxp3 pe, by Thermo Fisher Scientific (1 mentions) Foxp3 staining buffer, by Thermo Fisher Scientific (1 mentions) Collagenase 1, by Solarbio (1 mentions) Folate, by Bio Basic (1 mentions) Anti cd31 monoclonal antibody, by Abcam (1 mentions) Cd25 pecy5, by Thermo Fisher Scientific (1 mentions)

3 protocols using cxcr3 fitc

Chitosan-based EGFP-IP-10 Delivery System

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Chitosan (deacetylation degree ≥ 90%) was purchased from Solarbio Company (Beijing, China). Folic acid was purchased from Sigma-Aldrich (Sigma, USA). The recombinant IP-10 plasmid was constructed by cloning the mouse IP-10 cDNA fragment into the N-terminal of pReceiver-MO3, which contained the enhanced green fluorescent protein (EGFP) 8 (link). Monoclonal anti-mouse CD11b-FITC (m1-70) and Ly6G-PE (RB6-8C5), CD80-FITC (16-10A1), CD86-FITC (GL1), MHCII-FITC (NIMR-4), CXCR3-FITC, CD8-PE, and anti-mouse Ki67 were purchased from eBiosciences (San Diego, USA). Chitosanase was purchased from Calbiochem (Dames, Germany). Monoclonal rabbit anti-mouse IP-10 was acquired from Boster (Wuhan, China). Fluorescent apoptosis detection kit was obtained from Roche (Roche, Switzerland). Mouse IL-12 ELISA kit was from Peprotech (Peprotech, USA) and INF-γ ELISPOT assay kit was purchased from Dakewe (Shenzhen, China).

Hu Z., Chen J., Zhou S., Yang N., Duan S., Zhang Z., Su J., He J., Zhang Z., Lu X, & Zhao Y. (2017). Mouse IP-10 Gene Delivered by Folate-modified Chitosan Nanoparticles and Dendritic/tumor Cells Fusion Vaccine Effectively Inhibit the Growth of Hepatocellular Carcinoma in Mice. Theranostics, 7(7), 1942-1952.

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Splenocyte Isolation and Analysis

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Splenocytes were isolated from naïve mice, and from mice that had received Balb/c allografts from 4 groups; 1. B6 recipient, no treatment, 2. B6 recipient, treatment with 3mg/kg/day CsA, 3. B6 recipient, treatment with CR2-Crry, and 4. B6 recipient, treatment with CR2-Crry + 3mg/kg/day CsA. Spleens were isolated 7 days posttransplant. Isolated spleens were mechanical disrupted, suspensions passed through a series of nylon mesh strainers, and frozen in freezing media (Invitrogen) for later analysis. Splenocytes were thawed and stained for cell surface markers (CD3 eFluor450, CXCR3 FITC; eBioscience; CD4 APC Cy7, CD8 APC, CD44 PerCP Cy5.5, CD62L FITC, FoxP3 PE, BD Biosciences) and incubated in FACS buffer (PBS + 2% FBS) in the dark for 20 minutes at room temperature. Cells were then washed twice in FACS buffer and incubated in Fixation Buffer (BioLegend) for 10 minutes. After washing with FACS buffer, cells were run on a BDVerse (BD Biosciences) and analyzed using FlowJo software (Tree Star, Ashland, OR).

Zhu P., Bailey S.R., Lei B., Paulos C.M., Atkinson C, & Tomlinson S. (2017). Targeted Complement Inhibition Protects Vascularized Composite Allografts From Acute Graft Injury and Prolongs Graft Survival When Combined With Subtherapeutic Cyclosporine A Therapy. Transplantation, 101(4), e75-e85.

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Melanoma-Specific Peptide Protocol

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Chitosan (M_w 7 kDa, deacetylation degree > 95%) was purchased from Aoxing Bio-Technology Co. (Zhejiang, China). Folate was purchased from Bio Basic Inc. (Markham, ON, Canada). Monoclonal antibodies against mouse CD11b-FITC and Ly6G-PE were obtained from Beckman Coulter (Brea, CA, USA). Monoclonal antibodies against mouse CD4-FITC, CD8-PerCP-Cy5.5, CD25-PE-Cy5, CXCR3-FITC, Foxp3-PE and Foxp3 staining buffer were purchased from eBiosciences (San Diego, CA, USA). The anti-CD31 monoclonal antibody was obtained from Abcam (Cambridge, MA, USA). Purified hamster anti-mouse CD28 antibody was purchased from BD Biosciences (San Jose, CA, USA). The anti-mouse PCNA monoclonal antibody was supplied by Boster Bio-Technology Co. (Wuhan, China). TdT-mediated dUTP-biotin nick end labeling (TUNEL) cell-death fluorescein detection kit was obtained from Roche Diagnostics (Hoffmann-La Roche, Basel, Switzerland). Dynal mouse CD8⁺T isolation kit was purchased from Invitrogen (Carlsbad, CA, USA). Collagenase I was acquired from Solarbio Co. (Beijing, China). The H-2K^b-restricted, melanoma-specific antigenic peptide TRP2 (amino acids 180 - 188, N-SVYDFFVWL-COOH) was chemically synthesized by Nanjing GenScript Corporation (Nanjing, China) with a purity > 95%.

He J., Duan S., Yu X., Qian Z., Zhou S., Zhang Z., Huang X., Huang Y., Su J., Lai C., Meng J., Zhou N., Lu X, & Zhao Y. (2016). Folate-modified Chitosan Nanoparticles Containing the IP-10 Gene Enhance Melanoma-specific Cytotoxic CD8+CD28+ T Lymphocyte Responses. Theranostics, 6(5), 752-761.

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