The sesame meal extract was dissolved in 50% ethanol to 10 mg/mL, clarified with 0.45 µm syringe filters, and 10 μL of the sample was injected into a Shimadzu HPLC system (Shimadzu, Kyoto, Japan) with a COSMOSIL Cholester packed column (4.6 mm I.D. × 250 mm, Nacalai, Kyoto, Japan), eluted at 1 mL/min, 40 °C. A binary gradient elution was employed with 0.1% (v/v) acetic acid (Nacalai, Kyoto, Japan) aqueous solution and 95% acetonitrile (Nacalai, Kyoto, Japan) with 0.1% (v/v) acetic acid as mobile phase A and B, respectively. The gradient of mobile phase B was programed as: 0–5 min, 5% to 20%; 5–40 min, 20% to 60%; 40–50 min, 100%; 50–65 min, 5%. UV detection was carried out at 195 nm. The contents of free LA and OA in the sesame meal extract were quantified by using the standard curves generated with reference standards (Wako, Osaka, Japan).
Acetic acid
Manufactured by Nacalai Tesque
Sourced in Japan
Acetic acid is a clear, colorless liquid with a pungent odor. It has the chemical formula CH3COOH and is the main component of vinegar. Acetic acid is commonly used as a solvent, preservative, and for various chemical reactions in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Nacalai Tesque (2 mentions)
Ammonium acetate, by Nacalai Tesque (2 mentions)
Ethanol, by Nacalai Tesque (2 mentions)
Sodium hydroxide (naoh), by Nacalai Tesque (2 mentions)
N hexane, by Fujifilm (2 mentions)
Propionic acid, by Nacalai Tesque (2 mentions)
Hplc system, by Shimadzu (1 mentions)
β actin primary antibody, by Merck Group (1 mentions)
Alcian blue, by Merck Group (1 mentions)
1 2 dimethylhydrazine dmh, by Merck Group (1 mentions)
N n dimethyl p phenylenediamine, by Merck Group (1 mentions)
Methylene blue, by Nacalai Tesque (1 mentions)
Glyceraldehyde 3 phosphate dehydrogenase, by GeneTex (1 mentions)
Peroxidase affinipure goat anti mouse igg, by Abcam (1 mentions)
Pcna primary antibody, by Abcam (1 mentions)
Goat anti rabbit immunoglobulin g igg secondary antibody, by Abcam (1 mentions)
N n dimethyl m phenylenediamine, by Merck Group (1 mentions)
Isobutylmethylxanthine, by Merck Group (1 mentions)
Dulbecco s phosphate buffered saline dpbs, by Thermo Fisher Scientific (1 mentions)
Dmem low glucose, by Fujifilm (1 mentions)
22 protocols using acetic acid
1
HPLC Analysis of Sesame Meal Extract
2
HPLC Reagents Procurement Protocol
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Methanol, acetic acid, and distilled water for HPLC were purchased from Nacalai Tesque Inc., Kyoto, Japan. All other chemicals were reagent grade, and were purchased from Wako Pure Chemical Industries, Ltd., Tokyo, Japan or Nacalai Tesque Inc., Kyoto, Japan.
3
Anticancer Effects of Djulis Compound
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Djulis was obtained from Sinfong Agritech Co. (Taipei, Taiwan). Iron (III) chloride hexahydrate, methylene blue, and acetic acid were purchased from Nacalai Tesque (Tokyo, Japan), Showa Chemicals (Tokyo, Japan), and Shimakyu Pure Chemicals (Osaka, Japan), respectively. The Bax primary antibody and Bcl-2 primary antibody were purchased from Cell Signaling Technology (Beverly, MA, USA). Glyceraldehyde-3-phosphate dehydrogenase and the caspase-9 primary antibody were purchased from GeneTex (Irvine, CA, USA). The proliferating cell nuclear antigen (PCNA) primary antibody, goat anti-rabbit immunoglobulin G (IgG) secondary antibody, and peroxidase AffiniPure goat anti-mouse IgG were purchased from Abcam (Cambridge, UK), Southern Biotechnology (Birmingham, AL, USA), and Jackson ImmunoResearch (West Grove, PA, USA), respectively. The β-actin primary antibody, 1,2-dimethylhydrazine (DMH), N,N’-dimethyl-p-phenylenediamine, N,N’-dimethyl-m-phenylenediamine, Alcian blue, and the remaining chemicals were purchased from Sigma Chemical (St. Louis, MO, USA).
4
Regulation of Macrophage Oxidative Stress
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Human serum albumin (HSA) was purchased from the Japan Blood Products Organization (Tokyo, Japan). Diphenylene iodonium (DPI), MitoTEMPO, insulin, dexamethasone and isobutylmethylxanthine were purchased from Sigma-Aldrich (St Louis, MO, USA). Anti-F4/80 monoclonal antibody was purchased from eBioscience (San Diego, CA, USA). Potassium iodide, chloramine T, acetic acid and N-acetyl-L-cysteine (NAC) were purchased from Nacalai Tesque (Kyoto, Japan). 5-(and 6)-chloromethyl-2′,7′-dicholorodihydrofluorescein diacetate (CM-H2DCFDA) and Dulbecco’s phosphate-buffered saline (D-PBS) were purchased from Invitrogen (Grand Island, NY, USA). Dulbecco’s modified eagle medium (DMEM)-high glucose and DMEM-low glucose were purchased from FUJIFILM Wako Pure Chemical Co. (Osaka, Japan). All methods were carried out in accordance with approved guidelines. All experimental protocols were approved by Kumamoto University.
5
CBB Staining Protocol for Cell Imaging
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The CBB staining was performed as described previously.9 Briefly, the cells were washed once with 3 mL of Dulbecco's phosphate‐buffered saline (D‐PBS; Sigma). Subsequently, 2 mL of CBB stain [0.6% CBB (Thermo Fisher Scientific, Waltham, MA), 50% of methanol (Nacalai Tesque, Kyoto, Japan), 10% of acetic acid (Nacalai)] was added to the dish and stirred. Finally, the cells were washed three times with 5 mL of washing solution (50% of methanol and 10% of acetic acid) and dried at room temperature.
6
Zinc-Carboxylate Complex Solution Synthesis
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All the guaranteed reagents were used without further purification. Zinc sulfate heptahydrate (ZnSO4⋅7H2O) was purchased from KISHIDA CHEMICAL Co., Ltd., Japan, and acetic acid (AA), malonic acid (MA), and citric acid (CA) were purchased from Nacalai Tesque, INC. Japan. Solutions were prepared by dissolving the reagents in ultra-pure water. Two types of mother solutions were prepared, and they contained one of the carboxylic acids (AA, MA, CA) and Zn of different molar ratios. One contained 1.0 M acetate (Ac) and 0.5 M Zn, and the other contained 2.0 M Ac and 0.5 M Zn. Solutions of different pH values were prepared by adding small volumes of commercial 1.0 M hydrochloric acid or sodium hydroxide.
7
Fabrication of PCL-based Biocomposites
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For the production of the base material, 400 mg of PCL (Mw ~80,000, Sigma Aldrich, St. Louis, MO, USA) was added to 2 mL of acetic acid (Nacalai Tesque, Kyoto, Japan) and stirred for 12 h. For the composites, 5 mg, 10 mg, and 15 mg (about 1.2%, 2.4%, and 3.6%, respectively) of β-carotene powder (1 µm average particle diameter, Nacalai Tesque, Kyoto, Japan) were added to the solution and stirred for an additional hour.
Printing parameters were chosen from a previous work [33 (link)]: 1 mL of solution was added to a syringe with a 23G needle and injected at a rate of 0.2 μm/s (flow rate: 3.5 × 10−3 mm3/s) under an applied voltage of 10 kV while keeping the metal needle at a fixed distance of 5 cm from the target aluminum foil cathode. The samples were produced under controlled environmental conditions (T = 25 °C and RH = 25–35%).
Printing parameters were chosen from a previous work [33 (link)]: 1 mL of solution was added to a syringe with a 23G needle and injected at a rate of 0.2 μm/s (flow rate: 3.5 × 10−3 mm3/s) under an applied voltage of 10 kV while keeping the metal needle at a fixed distance of 5 cm from the target aluminum foil cathode. The samples were produced under controlled environmental conditions (T = 25 °C and RH = 25–35%).
8
Synthesis of Barium Carbonate Compounds
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An aqueous solution of formaldehyde (36 wt%), barium carbonate (BaCO3) and N,N,N′,N′-tetraethylethylenediamine were purchased from Sigma-Aldrich (St. Louis, MO). Calcium hydroxide (Ca(OH)2), silver(i ) oxide (Ag2O), alumina (Al2O3), imidazole, pyridine and acetylacetone were purchased from FUJIFILM Wako Pure Chemical Corp. (Osaka, Japan). Barium hydroxide octahydrate (Ba(OH)2·8H2O), sodium hydroxide (NaOH), potassium hydroxide (KOH), triethylamine, 2-picoline, 3-picoline, acetic acid and ammonium acetate were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Lithium oxide monohydrate (LiOH·H2O), 2-(dimethylamino)ethanol and acetonitrile for HPLC were purchased from Kishida Chemical Co., Ltd. (Osaka, Japan). Magnesium hydroxide (Mg(OH)2), 1,4-diazabicyclo[2.2.2]octane (DABCO) and N-methylpiperidine were purchased from Kanto Chemical Co. Inc. (Tokyo, Japan). Amberlite® IR-120 H and IRA-910 Cl were purchased from Sigma-Aldrich (St. Louis, MO). Amberlite® IRA-910 Cl was used after exchanging Cl− ions to OH− ions (Amberlite® IRA-910 OH) by washing with 1.0 M NaOH. For thin layer chromatography (TLC), Merck precoated TLC plates (silica gel 60 F254) were used. Water was purified with a Millipore Milli-Q system. Other reagents were used without further purification.
9
Chemical Preparation for Cell Culture
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PC (molecular weight, 581.56; purity, >95.1%), and DMDC-methyl (purity, 100%) were obtained from Wako Pure Chemical Industries Ltd. (Osaka, Japan). Na-DMDC (molecular weight, 143.21; purity, >98.0%) and 2Na-EBDC (molecular weight, 256.34; purity, 99.5%) were obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan) and Sigma–Aldrich (St. Louis, MO, USA), respectively. Stock solutions of PC, Na-DMDC, and 2Na-EBDC were prepared by diluting the compounds with dimethyl sulfoxide (DMSO; plant cell culture-tested DMSO, Sigma–Aldrich). Acetic acid was purchased from Nacalai Tesque Inc. (special grade, Kyoto, Japan). Other chemicals were obtained from Wako Pure Chemical Industries unless otherwise noted.
10
Tetraphenylporphyrin Synthesis and Characterization
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5,10,15,20-Tetrakis(4-aminophenyl)-21H,23H-porphyrin (Tph) and 4-ethylpyridine
(epy) were purchased from TCI (Tokyo, Japan). Acetic acid was purchased
from Nacalai Tesque (Kyoto, Japan). Cytochrome c from
bovine heart, TMB, 1,2-dichlorobenzene (DCB), and 2,5-dimethoxy-1,4-dibenzaldehyde
were purchased from Sigma-Aldrich (Missouri, USA). Other reagents
were purchased from FUJIFILM Wako (Tokyo, Japan). All chemicals were
used without further purification.
(epy) were purchased from TCI (Tokyo, Japan). Acetic acid was purchased
from Nacalai Tesque (Kyoto, Japan). Cytochrome c from
bovine heart, TMB, 1,2-dichlorobenzene (DCB), and 2,5-dimethoxy-1,4-dibenzaldehyde
were purchased from Sigma-Aldrich (Missouri, USA). Other reagents
were purchased from FUJIFILM Wako (Tokyo, Japan). All chemicals were
used without further purification.
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