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Jy2000 2

Manufactured by Horiba
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Sourced in Japan, France, United States

The JY2000-2 is a spectrometer designed for optical emission spectroscopy. It features a dual monochromator system with high resolution and accuracy. The device is capable of analyzing a wide range of samples and materials.

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Lab products found in correlation

Multimode 8, by Bruker (1 mentions) Spectrum two, by PerkinElmer (1 mentions) Cary 60 uv vis spectrophotometer, by Agilent Technologies (1 mentions) D8 advance, by Bruker (1 mentions) Jem 3200fs, by JEOL (1 mentions) Su8220, by Hitachi (1 mentions) H 800, by Hitachi (1 mentions) D max 2550 5, by Rigaku (1 mentions)

9 protocols using jy2000 2

1

Quantifying Mn2+ Release from Hydrogels

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The Mn2+ release profile from the Mn-based GG-MA hydrogels was quantified using inductively coupled plasma-optical emission spectroscopy (ICP; JY2000 2, Jobin Yvon, Horiba). Hydrogel cylinders, with 8 mm diameter and 2 mm height, were incubated in aCSF at 37 °C with mechanical shaking. At defined timepoints (0.5, 1, 5, 24, and 48 h), the aCSF supernatant was collected, dissolved in nitric acid and injected into the ICP equipment. Manganese (λem = 259.37 nm) concentrations in the aCSF solutions were obtained by comparison with standard solutions, with a detection limit of 5 ppb.
Vieira S., Strymecka P., Stanaszek L., Silva-Correia J., Drela K., Fiedorowicz M., Malysz-Cymborska I., Janowski M., Reis R.L., Łukomska B., Walczak P, & Oliveira J.M. (2023). Mn-Based Methacrylated Gellan Gum Hydrogels for MRI-Guided Cell Delivery and Imaging. Bioengineering, 10(4), 427.
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2

Quantification of AuNPs via ICP-OES

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Au concentration was determined via ICP-OES (JY 2000 2, HORIBA JOBIN YVON, Inc.), at 242.795 nm, after the dissolution of the AuNPs with 2.5% of HNO 3 and 5% of HCl. All measurements were carried out in triplicate.
Ferreira H ., Martins A ., Alves da Silva ML ., Amorim S ., Faria S ., Pires RA ., Reis RL , & Neves NM . (2018). The functionalization of natural polymer-coated gold nanoparticles to carry bFGF to promote tissue regeneration. Journal of materials chemistry. B, 6(14).
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3

Lithium Concentration Determination

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Liquid extracts were prepared according to the methods described above. Li+ concentrations in cell culture media at 1, 3, 5 and 7 days were determined by inductively coupled plasma–atomic emission spectroscopy (JY2000-2; Horiba Jobin Yvon, Kyoto, Japan).
Li L., Peng X., Qin Y., Wang R., Tang J., Cui X., Wang T., Liu W., Pan H, & Li B. (2017). Acceleration of bone regeneration by activating Wnt/β-catenin signalling pathway via lithium released from lithium chloride/calcium phosphate cement in osteoporosis. Scientific Reports, 7, 45204.
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4

Characterization of MnO2 Nanomaterials

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The morphologies of MnO2 nanomaterials were observed by high-resolution field emission transmission electron microscope (TEM) (JEM-3200FS, JEOL, Japan). The thickness of MNS was measured by atom force microscope (AFM) (MultiMode 8, Bruker, Germany). X-ray diffraction (XRD) pattern of MNS was detected by X-ray diffractometer (D8 Advance, Bruker, Germany). Dynamic light scattering (DLS) and Zeta potential was measured on Zetasizer Nano-ZS90 (Malven, England). Fourier-transform infrared (FT-IR) spectra were collected on a FT-IR spectrometer (Spectrum Two, PerkinElmer, USA). UV/Vis absorption spectra were measured on a Cary 60 UV/Vis spectrophotometer (Agilent Technologies, Santa Clara, CA, USA). The Mn element concentration of MNS was quantified by an inductively coupled plasma-atomic emission spectrometry (ICP-AES) (JY 2000-2, Horiba, France).
He T., Xu H., Zhang Y., Yi S., Cui R., Xing S., Wei C., Lin J, & Huang P. (2020). Glucose Oxidase-Instructed Traceable Self-Oxygenation/Hyperthermia Dually Enhanced Cancer Starvation Therapy. Theranostics, 10(4), 1544-1554.
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5

ICP-OES Analysis of Biosilica Ca and P

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Inductively coupled plasma-optical emission spectrometry (ICP-OES) was used to determine the Ca and P concentrations present in the SBF before and after the immersion of the biosilicas. The ICP-OES analysis was performed on a ICP spectrometer (HORIBA JY 2000-2, United States), using the absorption wavelengths of λ = 317.93 nm and λ = 214.91 nm for Ca and P, respectively. Concentrations of Ca and P were calculated from standard calibration curves prepared using standard solutions from 0 to 10 ppm.
Dudik O., Amorim S., Xavier J.R., Rapp H.T., Silva T.H., Pires R.A., & Reis R.L. (2021). Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges. Frontiers in Marine Science, 8.
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6

Comprehensive Sample Characterization

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The as-prepared samples were characterized by X-ray diffraction (XRD) with a X-ray diffractometer (Rigaku D/max 2,550 V, Cu Kα, λ=1.54178 Å), scanning electron microscope (SEM; Hitachi SU8220 and S-3400N; Hitachi, Tokyo, Japan), and transmission electron microscope (TEM; Hitachi H-800;). Ca, Mg, and P contents in the solution were measured using inductively coupled plasma (ICP)-optical emission spectrometer (JY 2000-2; Horiba).
Zhou D., Qi C., Chen Y.X., Zhu Y.J., Sun T.W., Chen F, & Zhang C.Q. (2017). Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects. International Journal of Nanomedicine, 12, 2673-2687.
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7

Batch Sorption of Rhenium from Aqueous Solutions

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All the sorption experiments were conducted using the batch sorption method. The solid/liquid ratio performed in all batch experiments was 0.5 g L−1. In a typical experiment, 4 mg of SCP-IHEP-1 or Cu-bpy was added into 8 mL of aqueous solution with a certain concentration of ReO4−. The pH values of the solutions were adjusted as required using NaOH and HNO3 and were measured on a digital pH-meter. The mixture was stirred for a specified time (t, min) at a specified temperature (T, K), and separated with a 0.22 μm nylon membrane filter. The concentrations of ReO4− in aqueous solution were determined by inductively coupled plasma optical emission spectrometry (ICP-OES, Horiba JY2000-2).
Mei L., Li F.Z., Lan J.H., Wang C.Z., Xu C., Deng H., Wu Q.Y., Hu K.Q., Wang L., Chai Z.F., Chen J., Gibson J.K, & Shi W.Q. (2019). Anion-adaptive crystalline cationic material for 99TcO4− trapping. Nature Communications, 10, 1532.
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8

pH-Responsive LCP Nanoparticle Leaching

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To examine the pH-sensitive gene release, the leaching of Ca2+ from the as-prepared LCP NPs was investigated. A typical experiment was performed as follows. About 10 mg of dry LCP NP powder was dispersed in 50 mL buffer solution with pH of 4.5, 6.5 and 7.4. Thereafter, the resulting suspension was shaken in a sealed vessel at 37°C with a constant shaking rate (140 rpm). The medium (0.4 mL) was withdrawn at given time points (0, 0.25, 0.5, 1, 2, 3, 4, and 24 h) and replaced with the same volume of fresh buffer with the same pH value. The withdrawn liquid was then filtered by 0.22 μm filter and the Ca2+ concentration was determined by inductively coupled plasma optical emission spectrometry (ICP-OES, JY2000-2, Horiba).
Tang J., Li L., Howard C.B., Mahler S.M., Huang L, & Xu Z.P. (2015). Preparation of optimized lipid-coated calcium phosphate nanoparticles for enhanced in vitro gene delivery to breast cancer cells. Journal of materials chemistry. B, Materials for biology and medicine, 3(33), 6805-6812.
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9

Elemental Composition Analysis of ACPs

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The elemental composition of the ACPs was determined using an ICP-OES (Horiba, JY2000-2). The samples were dissolved in 1% HNO3 solution for measurements. 5 solutions, containing a different concentration of Ca and P in each one, were prepared for the setup calibration.
Lu B.Q., Willhammar T., Sun B.B., Hedin N., Gale J.D, & Gebauer D. (2020). Introducing the crystalline phase of dicalcium phosphate monohydrate. Nature Communications, 11, 1546.
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