Methylone hcl

Pentylone⋅HCl and Methylone⋅HCl were obtained from Cayman Chemical. 3,4-methylenedioxymethamphetamine (MDMA) HCl was obtained from NIDA Drug Supply. The MDMA analog was obtained from Fox Chase Chemical Diversity Center (Doylestown, PA, United States). Drugs were dissolved in physiological saline for the i.v. routes of administration. Dosing is expressed as the salt.
We purchased tetrodotoxin (TTX) from Biotium (Hayward, CA, United States), and AP-5, CGP55845A and DNQX, U-50488, and nor-binaltorphimine from Tocris (Bristol, United Kingdom) for the electrophysiological recordings. Stock solutions of the drugs were prepared in either distilled water or dimethyl sulfoxide (DMSO) and added to the bath solution to achieve the desired concentration.

Methylone HCl (0.5–30 mg/kg; Cayman Chemical) or Fluoxetine HCl (10 mg/kg; Sigma-Aldrich) were formulated in sterile 0.9% saline vehicle before IP administration. Control animals received saline vehicle. Methylone or saline was administered 30 min prior to FST or OFT testing sessions. Fluoxetine or saline were administered 23.5, 5, and 1 h prior to testing, consistent with previous studies (19 (link)).