Log In Sign up
The largest database of trusted experimental protocols

Oxylipin standards

Manufactured by Cayman Chemical
Visit Supplier

Oxylipin standards are reference materials used for the identification and quantification of oxylipins in samples. Oxylipins are a class of bioactive lipid mediators derived from the oxidation of polyunsaturated fatty acids. These standards provide a reliable and consistent way to analyze oxylipin levels in biological and chemical samples.

Automatically generated - may contain errors

Lab products found in correlation

P egfr, by Cell Signaling Technology (1 mentions) P src, by Cell Signaling Technology (1 mentions) Cyp3a, by Santa Cruz Biotechnology (1 mentions) Flotillin 1, by Santa Cruz Biotechnology (1 mentions) P fak, by Cell Signaling Technology (1 mentions) Hexane, by Thermo Fisher Scientific (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Glycerol, by Merck Group (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Sodium carbonate, by Merck Group (1 mentions) Chloroform, by Thermo Fisher Scientific (1 mentions) Ethyl acetate, by Thermo Fisher Scientific (1 mentions) Toluene, by Thermo Fisher Scientific (1 mentions) Butylated hydroxytoluene, by Merck Group (1 mentions) Ethylenediaminetetraacetic acid edta, by Merck Group (1 mentions) Triphenylphosphine, by Merck Group (1 mentions) Acetic acid, by Merck Group (1 mentions) Complete protease inhibitor tablet, by Roche (1 mentions) Acetic acid, by Thermo Fisher Scientific (1 mentions) Ammonium hydroxide, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Oxylipin (5 citations) Oxylipin standards and internal standards (2 citations)

5 protocols using oxylipin standards

1

Oxylipin Analysis and Signaling Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals and solvents were used as purchased. Oxylipin standards were purchased from Cayman Chemicals (Ann Arbor, MI). Antibodies used for western blotting and immunohistochemical staining were: FAK, p-FAK, Src, p-Src, EGFR, and p-EGFR obtained from Cell Signaling Technology (Beverly, MA); and CYP2B, CYP2C9/19, CYP2J, CYP3A, and flotillin-1 purchased from Santa Cruz (Santa Cruz, CA).
Apaya M.K., Shiau J.Y., Liao G.S., Liang Y.J., Chen C.W., Yang H.C., Chu C.H., Yu J.C, & Shyur L.F. (2019). Integrated omics-based pathway analyses uncover CYP epoxygenase-associated networks as theranostic targets for metastatic triple negative breast cancer. Journal of Experimental & Clinical Cancer Research : CR, 38, 187.
+ Open protocol
+ Expand
2

Hepatic lipid and metabolite analysis

Cited 4 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Hepatic lipids were extracted and process for gas chromatography (GC), ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UPLC/MS/MS) and liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS) as described [43 (link),47 (link)]. Fatty acid standards for the GC analysis were purchased from Nu-Chek Prep and Cayman Chemical Company. Oxylipin standards were purchased from Cayman Chemical Company [47 (link)]. Hepatic glutathione (GSH and GSSG) and selected metabolites were fractionated and quantified using the metabolomic approach described previously [57 (link)], while hepatic bile acids were fractionated and quantified as described [58 (link)].
Spooner M.H., Garcia-Jaramillo M., Apperson K.D., Löhr C.V, & Jump D.B. (2023). Time course of western diet (WD) induced nonalcoholic steatohepatitis (NASH) in female and male Ldlr-/- mice. PLOS ONE, 18(10), e0292432.
+ Open protocol
+ Expand
3

Oxylipin Profiling and Fatty Acid Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Methanol, ethyl acetate, chloroform, toluene, and hexane were obtained from Fisher Scientific (Hampton, NH). Methanol was LC-MS grade, whereas all other solvents were HPLC grade. Acetic acid, butylated hydroxytoluene, sodium carbonate, glycerol, triphenylphosphine, ethylenediaminetetraAcetic acid (EDTA), and hydrochloric acid were purchased from Sigma-Aldrich (St. Lous, MO). Oxylipin standards were purchased from Cayman Chemicals (Cayman Chemical, Ann Arbor, MI) or synthesized by Dr. Bruce Hammock’s lab at UC Davis. The synthetic standards included the following ALA-derived oxylipins: 15(16)-EpODE, 12(13)-EpODE, 9(10)-EpODE, 15,16-DiHODE, 12,13-DiHODE and 9,10-DiHODE. Fatty acid standards were purchased from NuCheck Prep (Elysian, MN). Oils were purchased from local stores.
Richardson C.E., Hennebelle M., Otoki Y., Zamora D., Yang J., Hammock B.D, & Taha A.Y. (2017). Lipidomic quantitation of oxidized fatty acids in plant and algae oils. Journal of agricultural and food chemistry, 65(9), 1941-1951.
+ Open protocol
+ Expand
4

Oxylipin Extraction and Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Oxylipin Standards were purchased from Cayman Chemical (Ann Arbor, Michigan) or Biomol (Plymouth Meeting, PA). Oasis HLB (Hydrophilic-Lipophilic Balanced) solid phase extraction cartridges were obtained from Waters (Milford Massachusetts). Acetic acid, water (HPLC grade), methanol (LC/MS grade), acetonitrile (LC/MS grade) and ammonium hydroxide were obtained from Fisher Scientific (Fair Lawn, New Jersey). cOmplete Protease Inhibitor tablets were from Roche. Irradiated control and ethanol Lieber-DeCarli Diets were from Bio-Serv (Frenchtown, NJ).
, & Barrington K.J. (2001). The adverse neuro-developmental effects of postnatal steroids in the preterm infant: a systematic review of RCTs. BMC Pediatrics, 1, 1.
+ Open protocol
+ Expand
5

Oxylipin Analytical Workflow Using LC-MS

Check if the same lab product or an alternative is used in the 5 most similar protocols
Oxylipin standards were obtained from Cayman Chemical (Ann Arbor, MI). All lipid substrates were dissolved in methanol and combined to form stock solution mixes (1 μg mL−1) and stored under nitrogen at −20 °C until use. Water was either 18 mQ, or Omnisolv LC-MS grade (MilliporeSigma, Oakville, ON, Canada). HPLC and HiPerSolve LC-MS grade methanol and acetonitrile were purchased from VWR (Radnor, PA). Nano-TiO2 powder was obtained from Evonik (P25 Aeroxide TiO2, Parsippany, NJ) and was composed of a mixture of anatase (80%) and rutile (20%) TiO2. Particle characteristics have previously been determined, including primary particle size (21 nm), specific surface area (48.08 m2 g−1), and zeta potential (−56.6 mV).23,24
Harris T.R., Griffith J.A., Clarke C.E., Garner K.L., Bowdridge E.C., DeVallance E., Engles K.J., Batchelor T.P., Goldsmith W.T., Wix K., Nurkiewicz T.R, & Rand A.A. (2023). Distinct profiles of oxylipid mediators in liver, lung, and placenta after maternal nano-TiO2 nanoparticle inhalation exposure. Environmental Science. Advances, 2(5), 740-748.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!