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Cd49d fitc

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CD49d FITC is a fluorescently-labeled antibody that binds to the CD49d (alpha 4 integrin) cell surface antigen. It can be used to detect and analyze cells expressing CD49d in flow cytometry applications.

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Lab products found in correlation

Facscanto 2, by BD (1 mentions) Facsdiva software, by BD (1 mentions) Ter119 pe, by Thermo Fisher Scientific (1 mentions) Cd49e pe, by Thermo Fisher Scientific (1 mentions) Streptavidin pe, by Thermo Fisher Scientific (1 mentions) Cd4 pe, by Thermo Fisher Scientific (1 mentions) Cd3 fitc, by Thermo Fisher Scientific (1 mentions) Cd11b fitc, by Thermo Fisher Scientific (1 mentions) Cd8 apc, by Thermo Fisher Scientific (1 mentions) Cd24 pe cy7, by BioLegend (1 mentions) Cd3 pe, by BioLegend (1 mentions) B220 apc, by BioLegend (1 mentions) Streptavidin pe cy7, by BioLegend (1 mentions) Cd45 bv510, by BioLegend (1 mentions) Cd69 pe cy7, by BioLegend (1 mentions) B220 apc cy7, by BioLegend (1 mentions) Ly6g pe, by BioLegend (1 mentions) Cd11c pe cy7, by BioLegend (1 mentions) Igm fitc, by Thermo Fisher Scientific (1 mentions) Cd4 percp cy5, by Thermo Fisher Scientific (1 mentions)

2 protocols using cd49d fitc

1

Erythroid Cell Development Analysis

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Bone marrow, spleen and fetal liver cells were stained for erythroid subsets using the antibodies listed below. Samples were analyzed by a FACSCantoII flow cytometer using FACSDiva software (Becton-Dickinson). Erythrocyte development in the bone marrow and spleen was analyzed as follows: Ter119medCD71high (proerythroblasts; R1), Ter119highCD71high (basophilic erythroblasts; R2), Ter119highCD71med (late basophilic and polychromatophilic erythroblasts; R3) and Ter119highCD71low (orthochromatophilic erythroblasts; R4). Cells were stained with the fluorescently labeled antibodies: Ter119-PE (eBioscience), CD71 Biotin (eBioscience), Streptavidin eflour 780 (eBioscience), CD49e PE (integrin α5), and CD49d FITC (integrin α4).
Uras I.Z., Scheicher R.M., Kollmann K., Glösmann M., Prchal-Murphy M., Tigan A.S., Fux D.A., Altamura S., Neves J., Muckenthaler M.U., Bennett K.L., Kubicek S., Hinds P.W., von Lindern M, & Sexl V. (2017). Cdk6 contributes to cytoskeletal stability in erythroid cells. Haematologica, 102(6), 995-1005.
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2

Multicolor Flow Cytometry Panel

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Commercial antibodies (clones, fluorophores and sources) are as follows: CD3-FITC (145–2C11), CD62L-PE (Mel-14), CD4-PErCP/Cy5.5 (RM4–5), CD8-PerCP/Cy5.5 (53–6.7), CD8-APC (53–6.7), CD209b-APC (22D1), IgM-FITC (II/41), CD4-PE (GK1.5), CD25-AF488 (PC61.5), Streptavidin-PE, CD169-PE/Cy7 (3D6.112) (eBioscience, San Diego, CA); CD11b-FITC (M1/70), CD49d-FITC (R1–2), TCRβ-FITC (GL3), CD21/35-PE (7E9), CD23-APC (B3B4), Ly6G-PE (RB6–8C5), Ly6C-PerCP/Cy5.5 (HK1.4), CD11c-PE/Cy7 (N418), B220-PerCP/Cy5.5 (RA3–6B2), B220-APC (RA3–6B2), B220-APC/Cy7 (RA3–6B2), CD69-BV421 (H1.2F3),CD23-Biotin (B3B4), F4/80-APC (BM8), Ly6G-BV421 (1A8), CD45-Pacific Blue (30-F11), CD45-BV510 (30-F11), CD43-PE (1B11), CD24-PE/Cy7 (M1/69), IgD-Pacific Blue (11–26c.2a), CD69-PE/Cy7 (H1.2F3), IgD-PerCP/Cy5.5 (11–26c.2a), Ly51-AF647 (6C3), CD3-Pacific Blue (17A2), CD3-PE (17A2), TCRγ/δ-biotin (GL3), Streptavidin-PE/Cy7 (Biolegend, San Diego, CA); Siglec-F-PE (E50–2440) (BD Biosciences, San Jose, CA). Samples were preincubated with 1 μg Fc-block (2.4G2 hybridoma; ATCC).
Cells were acquired either on the BD Biosciences LSR Fortessa or with a BD FACScan flow cytometer with DxP multi-color upgrades by Cytek Development Inc. (Woodland Park, NJ) and analyzed using FlowJo software (FlowJo LLC, Ashland, OR).
Anaya E.P., Lin X., Todd E.M., Szasz T.P, & Morley S.C. (2021). Novel mouse model reveals that serine phosphorylation of L-plastin is essential for effective splenic clearance of pneumococcus. Journal of immunology (Baltimore, Md. : 1950), 206(9), 2135-2145.
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