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2 protocols using β d galactose pentaacetate

1

Carbohydrate-Functionalized Carbon Nanotube Bioconjugates

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The SWNTs were obtained from Unidym (HiPco, Lot# P2150). Methyl pentafluorobenzoate, D-(+)-mannose, β-D-(+)-galactose pentaacetate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), sodium metabisulfide (Na2S2O5) and palladium (10% on carbon) were purchased from Alfa Aesar. Boron trifluoride ethyl etherate (BF3·Et2O), N-hydroxysuccinimide (NHS), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDAC·HCl), 1, 2-dichlorobenzene (DCB), and bovine serum albumin (BSA) were obtained from Sigma-Aldrich. Sodium azide (NaN3) and sodium methoxide (CH3ONa) were acquired from Fluka. 2-[2-(2-Chloroethoxy)-ethoxy]ethanol was obtained from TCI Japan. Concanavalin A (fluorescein conjugate, lectin from Jack bean) was purchased from Molecular Probes (Eugene, Oregon). All chemicals were used as received without purification. Water used was from a Milli-Q ultrapure water purification system. PFPA-NHS was synthesized following previously reported procedures.40 (link), 41 (link) Man-EG2-NH2 and Gal-EG2-NH2 were synthesized following previously established procedures (see Supporting Information for detailed synthesis and characterization).42 (link)–44
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2

Synthesis and Evaluation of Stimuli-Responsive Nanomedicines

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β-D-Galactose pentaacetate (98%) was purchased from Alfa Aesar (China) Chemical Co. Ltd. 2-Hydroxyethyl methacrylate (HEMA, 97%) was obtained from J&K Scientific Ltd. (China). Boron trifluoride diethyletherate (BF3⋅Et2O, 98%) was purchased from Aladdin Reagent (Shanghai) Co., Ltd. 4-Cyano-4-(phenylcarbonothioylthio)pentanoic acid (CPA, >97%), GSH, and 2,2′-Azobis(2-methylpropionitrile) (AIBN) were purchased from Sigma-Aldrich (China) Inc. 4-Mercaptophenylboronic acid (MPBA, >95%) was obtained from Energy Chemical Co. (China). DOX hydrochloride (DOX⋅HCl, 99%) was provided by Beijing HvsF United Chemical Materials Co., Ltd. (China) and used as received. AIBN was recrystallized from ethanol before use. All other chemical regents and solvents were purchased from Shanghai Chemical Reagent Co. (China) and of analytical reagent grade, used directly. Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute (RPMI) 1640 medium, fetal bovine serum (FBS), penicillin-streptomycin, and Lysotracker green DND-26 were purchased from Thermo Fisher Scientific (United States). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), trypsin, and 4,6-diamidino-2-phenylindole (DAPI) staining solution were obtained from Beyotime Biotechnology Co., Ltd. (China).
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