Cells grown on coverslips were rinsed in PBS, fixed in 4% (v/v) paraformaldehyde in PBS, permeabilised using 0.2% (v/v) Triton-X100 in PBS, blocked in 3% (w/v) BSA in PBS containing 0.1% Tween-20 (PBST) and incubated with primary antibody diluted in blocking buffer. Secondary detection utilised goat anti-mouse/rabbit IgG labelled with Alexa Fluor 488/594 (Life Technologies). F-actin was stained using 5 μM TRITC-phalloidin (Sigma-Aldrich). DNA was stained using 1 μM DAPI. Coverslips were mounted using ProLong® Gold antifade (Life Technologies). Confocal images were captured on a Zeiss 510 Inverted Confocal microscope using 63× objective lense.
Goat anti mouse rabbit igg labelled with alexa fluor 488 594
Manufactured by Thermo Fisher Scientific
Goat anti-mouse/rabbit IgG labeled with Alexa Fluor 488/594 is a secondary antibody used for detecting and visualizing mouse or rabbit primary antibodies in various applications such as immunofluorescence, flow cytometry, and Western blotting. The Alexa Fluor 488 or 594 dye used for labeling provides bright and photostable fluorescent signals.
Automatically generated - may contain errors
3 protocols using goat anti mouse rabbit igg labelled with alexa fluor 488 594
1
Immunofluorescence Staining of Cells
2
Immunofluorescence Staining for Confocal Microscopy
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For confocal microscopy, cells were cultured on coverslips, rinsed in PBS and fixed in 4% (v/v) paraformaldehyde in PBS. Subsequently, the samples were permeabilised using 0.2% (v/v) Triton X-100 in PBS, blocked in 3% (w/v) bovine serum albumin in PBS containing 0.1% Tween-20 (PBST) and incubated with a primary antibody diluted in blocking buffer. For secondary detection, goat anti-mouse/rabbit IgG labelled with Alexa Fluor 488/594 (Life Technologies) was used. DNA was stained using 1 μM DAPI (4′,6-diamidino-2-phenylindole). Coverslips were mounted using ProLong Gold antifade (Life Technologies). Confocal images were captured on a Zeiss 510 inverted confocal microscope using a Plan Apochromat 63 × 1.40NA oil immersion objective lens (Carl Zeiss GmbH, Jena, Germany).
3
Immunofluorescence Staining of Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells grown on coverslips were rinsed in PBS, fixed in 4% (v/v) paraformaldehyde in PBS, permeabilised using 0.2% (v/v) Triton-X100 in PBS, blocked in 3% (w/v) BSA in PBS containing 0.1% Tween-20 (PBST) and incubated with primary antibody diluted in blocking buffer. Secondary detection utilised goat anti-mouse/rabbit IgG labelled with Alexa Fluor 488/594 (Life Technologies). F-actin was stained using 5 μM TRITC-phalloidin (Sigma-Aldrich). DNA was stained using 1 μM DAPI. Coverslips were mounted using ProLong® Gold antifade (Life Technologies). Confocal images were captured on a Zeiss 510 Inverted Confocal microscope using 63× objective lense.
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