Eosinophils from murine intestines were cultured at 37℃ and 5% CO2 in RPMI containing 1% penicillin/streptomycin and 10% FBS in 96-well at a cell concentration of 2.0×104 cells/well. Eosinophils were cultured in the presence of recombinant interleukin 5 (IL-5) (1 ng/mL; R&D Systems, Minneapolis, MN, USA) and dexamethasone concentration (0 to 100 nM), and survival rate in the presence of dexamethasone was compared to viability in recombinant IL-5 medium alone. Viable cells were assessed by the trypan blue exclusion method. Cell viability was observed at day 3 for all experiments and was calculated by the total cell count minus the count of nonviable or dead cells.
Recombinant il 5
Manufactured by R&D Systems
Sourced in United States
Recombinant IL-5 is a protein produced using recombinant DNA technology. It is a cytokine that plays a role in the differentiation and activation of eosinophils, a type of white blood cell. This product is intended for research use only.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using recombinant il 5
1
Eosinophil Survival Modulation by Dexamethasone
2
Eotaxin-1 and IL-5 Interaction Assay
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Recombinant human Eotaxin-1 (R&D systems) and recombinant IL-5 (R&D systems) at an amount of 50 ng/sample were incubated overnight in kit specific reaction buffer with 1 μg/ml rSmCI-1, 1 μg/ml rSmCI-1Mut, or 1 μg/ml trypsin. Western blotting was performed as detailed above, using a 1:1000 (0.3 μg/ml) dilution of monoclonal mouse anti-Eotaxin-1 or IL-5 (R&D).
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