cDNA from the HAEC RNA aliquots were synthesized using SuperScript IV First-Strand Synthesis System (Invitrogen). Quantitative real-time RT-PCR, used to confirm selected NanoString findings, was performed on this cDNA using iQTM SYBER Green Supermix (Bio-Rad Laboratories, Hercules, CA) according to the manufacturer’s recommendation. The primers used in these reactions were produced by Integrated DNA Technologies (Skokie, IL) and included IL-8 forward (5′-GACCACACTGCGC CAACAC-3′) and reverse (5′-CTTCTCCA CAACCCTCTGCAC-3′) and IFN regulatory factor-7 (IRF-7) forward (5′-ACCCTGG CTGTGCCGAGT-3′) and reverse (5′-AAGCACTCGATGTCGT CATAGAG-3′). GAPDH primers were purchased from Bio-Rad Laboratories. Reactions were performed in triplicate and normalized to GAPDH using the comparative 2−ΔΔCT method (28 (link)).
Gapdh primers
Manufactured by Bio-Rad
GAPDH primers are a set of oligonucleotide sequences designed for the detection and quantification of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. GAPDH is a widely used housekeeping gene that is involved in the glycolytic pathway and is commonly used as a reference gene for gene expression studies.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using gapdh primers
1
Gene Expression Analysis of HAEC Cells
2
Gene Expression Analysis of HAEC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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cDNA from the HAEC RNA aliquots were synthesized using SuperScript IV First-Strand Synthesis System (Invitrogen). Quantitative real-time RT-PCR, used to confirm selected NanoString findings, was performed on this cDNA using iQTM SYBER Green Supermix (Bio-Rad Laboratories, Hercules, CA) according to the manufacturer’s recommendation. The primers used in these reactions were produced by Integrated DNA Technologies (Skokie, IL) and included IL-8 forward (5′-GACCACACTGCGC CAACAC-3′) and reverse (5′-CTTCTCCA CAACCCTCTGCAC-3′) and IFN regulatory factor-7 (IRF-7) forward (5′-ACCCTGG CTGTGCCGAGT-3′) and reverse (5′-AAGCACTCGATGTCGT CATAGAG-3′). GAPDH primers were purchased from Bio-Rad Laboratories. Reactions were performed in triplicate and normalized to GAPDH using the comparative 2−ΔΔCT method (28 (link)).
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