Sequencing libraries were prepared following the SureSelect version 1.7 target enrichment system (Agilent, Santa Clara, CA, USA) [33 (link)]. Briefly, 50 μL of DNA was sheared acoustically using an LE220 sonicator (Covaris, Brighton, UK). Fragmented DNA was end-repaired, A-tailed, and adaptor-ligated using the KAPA LTP library preparation kit (KAPA Biosystems, Wilmington, DE, USA). The resultant HCMV-enriched libraries were then indexed and sequenced on a MiSeq (Illumina, San Diego, CA, USA) with v3 chemistry to yield 300 × 300 nt paired-end reads datasets.
Sureselect version 1.7 target enrichment system
Manufactured by Agilent Technologies
Sourced in United States
The SureSelect version 1.7 target enrichment system is a laboratory equipment product designed for selective amplification and sequencing of targeted genomic regions. The system enables researchers to focus on specific areas of interest within the genome, improving the efficiency and cost-effectiveness of sequencing projects.
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Lab products found in correlation
2 protocols using sureselect version 1.7 target enrichment system
1
HCMV Enrichment Library Preparation
2
SureSelect Target Enrichment for Illumina Sequencing
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The SureSelect version 1.7 target enrichment system (Agilent) was used to prepare sequencing libraries (Supplementary Table 1 [rows 8–10]) [22 (link)]. These were sequenced using a MiSeq (Illumina) with version 3 chemistry generating original datasets of paired-end reads of 300 nucleotides (nt) (Table 1 and Supplementary Table 1 [rows 11–12]).
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