Generation of the BaP co‐exposure with Der f 1‐induced asthma mouse model was established as previously described.22 , 23 Briefly, mice were sensitized and challenged every other week for 6 weeks with intranasal administrations of 25 µg Der f 1 (Indoor biotechnologies) under light isoflurane anesthesia. To assess the role of BaP on Der f 1‐induced allergic airway inflammation, BaP (Sigma‐Aldrich) was dissolved in borate saline buffer (BBS, Sigma‐Aldrich), and intranasally administered at a concentration of 1 or 20 pmol once every week during Der f 1 sensitization and challenge (Figure 1 A). In some cases, mice were pretreated with CH223191 at a dose of 5 mg/kg or N‐acetyl‐L‐cysteine (NAC) at a dose of 1 mmol/kg dissolved in buffered saline by means of intraperitoneal administration 1 hour before BaP treatment. Age‐ and gender‐matched control mice were treated identically with BBS.
Der f 1
Manufactured by Indoor Biotechnologies
Sourced in United Kingdom
Der f 1 is a laboratory tool for the detection and measurement of Der f 1, a major allergen found in house dust mites. It is designed to provide accurate and reliable quantitative data on Der f 1 levels in environmental samples.
Automatically generated - may contain errors
4 protocols using der f 1
1
Bap Co-exposure with Der f 1-Induced Asthma Model
2
Asthma Mouse Model Generation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The generation of an asthma mouse model was established as previously described (29 (link)). Briefly, both male and female mice were sensitized and challenged every other week for 6 weeks with intranasal administrations of 25 μg Der f 1 (Indoor Biotechnologies) under isoflurane anesthesia. BaP (Sigma-Aldrich) was dissolved in borate saline buffer (Sigma-Aldrich) and intranasally administered at a concentration of 20 μM once every week during Der f 1 sensitization and challenge. In some cases, the mice were pre-treated with Fasudil or Y-27632 dissolved in 0.5% DMSO in phosphate-buffered saline (PBS) at a dose of 30 mg/ml by intraperitoneal administration 1 h before every single BaP treatment. Age- and gender-matched control mice were treated with PBS.
3
Measurement of Total IgE, IgG1, and Specific IgE
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total IgE and IgG1 were assayed by ELISA in serum. Specific anti-Der f 1 IgE and anti-OVA IgE were assessed by indirect ELISA. 96 wells plates were coated with sodium bicarbonate 50 μM and 0.25 μg of purified Der f 1 (Indoor Biotechnologies, Wiltshire, UK) or 5 μg OVA (Sigma-Aldrich, L'Isle d'Abeau Chesnes, France) and left overnight at 4°C. Wells were then incubated with bovine serum albumine 1% for 12 h at 4°C, washed, filled with sera diluted in CGS1 (Cosmo Bio, California, USA) and incubated overnight at 4°C. Specific Ig was revealed with anti-mouse IgE (AbD serotec, Colmar, France) coupled to HRP. Substrate ABTS (Roche, Boulogne-Billancourt, France) was added and absorbance measured at 405 nm with VictorTMX3 (PerkinElmer, Courtaboeuf, France).
4
Proteases from Diverse Plant Sources
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The PLCPs analysed in this study are from D. pteronyssinus and D. farinae (Der p 1 and Der f 1, Indoor Biotechnologies, Cardiff, UK), C. papaya, (Cari p Papain and Cari p 2, Sigma-Aldrich, Milan, Italy), A. comosus, (Ana c 2, Sigma-Aldrich), and F. carica (Fic c Ficin, Sigma-Aldrich). Act d 1 was purified from A. deliciosa, following an already reported procedure [9 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!