The stachyose (pure>80%) was extracted from soybean residue [22 (link)]. Soybean isoflavones (pure>95%) includes 50% of daidzin, 6% of daidzein, 26% of glycitin, 3% of glycitein, 10% of genistin and 2% of genistein for animal experiments and the analytical standards of daidzein, glycitein and genistein (pure>98%) were bought from Shanghai Yuanye Biotechnology co., Ltd. (Shanghai, China). Assay kits of total cholesterol (TC), total triglyceride (TG), high-densitylipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), glutamic-oxal(o)acetic transaminase (GOT) and glutamicpyruvic transaminase (GPT) were purchased from HuiliBiotechnology Co. Ltd. (Changchun, China). Additionally, enzyme linked immunosorbent assay ELISA kits of interleukin-1 (IL-1), tumor necrosis factor (TNF-α), nuclear factor kappa-B (NF-κB), acetyl CoA carboxylase (ACC), fatty acid synthetase (FAS), glucose, insulin, leptin and lipopolysaccharide (LPS) were purchased from Jiancheng Bioengineering Institute (Nanjing, China). All other reagents and chemicals were analytical grade or higher.
Genistein
Manufactured by Yuanye Bio-Technology
Sourced in China
Genistein is a laboratory product manufactured by Yuanye Bio-Technology. It is a chemical compound that serves as a core component for various research and analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Daidzin, by Yuanye Bio-Technology (8 mentions)
Genistin, by Yuanye Bio-Technology (8 mentions)
Daidzein, by Yuanye Bio-Technology (8 mentions)
Glycitin, by Yuanye Bio-Technology (5 mentions)
Glycitein, by Yuanye Bio-Technology (5 mentions)
Ethanol, by Xilong (3 mentions)
Methanol, by Xilong (3 mentions)
Formic acid, by Xilong (3 mentions)
Ethylene glycol, by Xilong (3 mentions)
Quercetin, by Yuanye Bio-Technology (2 mentions)
Ferulic acid, by Yuanye Bio-Technology (2 mentions)
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Puerarin, by Yuanye Bio-Technology (2 mentions)
Ononin, by Yuanye Bio-Technology (2 mentions)
Biochanin a, by Yuanye Bio-Technology (2 mentions)
Syringic acid, by Yuanye Bio-Technology (1 mentions)
Ascorbic acid, by Yuanye Bio-Technology (1 mentions)
Epicatechin, by Yuanye Bio-Technology (1 mentions)
Vanillic acid, by Yuanye Bio-Technology (1 mentions)
Isorhamnetin, by Yuanye Bio-Technology (1 mentions)
12 protocols using genistein
1
Extraction and Purification of Soybean Compounds
2
Soybean Fermentation with R. oligosporus
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The high purity (≥97%) standards, including ascorbic acid, vanillic acid, syringic acid, epicatechin, daidzin, glycitin, ferulic acid, genistin, daidzein, glycitein, quercetin, and genistein were purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Other reagents of analytical grade. Soybeans (Liaodou, yellow seed coat, mainly planted in northern China, weigh 21.6 g of one hundred seeds) were obtained from a local market. R. oligosporus RT-3 isolated, screened and identified from Tempeh in our laboratory was used for soybean fermentation (11 (link), 12 ). The strain was cultivated on potato dextrose agar at 30°C for 3 days. Then, spores produced were scraped in water (1 × 107 spores/mL).
3
Metabolite Sources for Biomedical Research
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Metabolites in NDS including isorhamnetin (YA0422YA14), genistein (Y25M6Y1), citric acid (B21313), and ursolic acid (B21403) were provided by Yuanye Biotechnology Co., Ltd. (Shanghai, China); kaempferol (20090615) was provided by Huike Plant Development Co., Ltd. (Shanxi, China); quercetin (117,395) was provided by Yaoyuan Biotechnology Co., Ltd. (Chengdu, China); daidzein (RSD-070426) was provided by Renshou Pharmaceutical Co., Ltd.; ferulic acid (20071006) was provided by Baicao Plant Co., Ltd. (Xuancheng, China); and puerarin (20070723) was provided by Shifang Jubang Plant Raw Materials Co., Ltd. (Deyang, China). Acetylcholine (ACh) and ET-1 were purchased from Sigma, United States. Other reagents are all domestically pure and meet the requirements of each experiment.
4
Phytochemical Analysis of Pueraria Extracts
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Folin-Ciocalteu's reagent, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2′-Azobis(2-methylpropionamidine) dihydrochloride (AAPH), fluorescein sodium salt, 2′,7′-dichlorofluorescin diacetate (DCFH-DA), Tris(hydroxymethyl)methyl aminomethane, β-Nicotinamide Adenine Dinucleotide (β-NAD), β-Mercatoethanol (β-Me) and α-D-glucopyranoside (pNPG) were obtained from Sigma-Aldrich (St. Louis, MO, USA). The enzymes used, including alcohol dehydrogenase (ADH) from saccharomyces cerevisiae, potassium-activated aldehyde dehydrogenase (ALDH) from yeast, α-glucosidase from saccharomyces cerevisiae, and α-amylase from porcine pancreas were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Standard phenolic compounds including 3′-hydroxypuerarin, 6″-O-xylosidepuerarin, puerarin, mirificin, 3″-methyoxypuerarin, daidzin, rutin, ferulic acid, genistin, phlorizin, ononin, daidzein, genistein, quercitrin, and p-coumaric acid were obtained from Yuanye Biotechnology Co., Ltd. (Shanghai, China). HPLC-grade acetic acid and acetonitrile were purchased from Fisher Scientific (Waltham, MA, USA). All other reagents used were of analytical grade.
5
Genistein Cytotoxicity Assay in RAW264.7 Cells
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RAW264.7 cells were inoculated into 96-well plates with 4 × 103 cells per well. Add 100 µL (3.125, 6.25, 12.5, 25, 37.5, 50, 75, 100 µmol/L) Genistein (Yuanye, Shanghai, China) to each well. The control group was added with DMEM complete medium. After 24 h culture, 10 µL CCK-8 solution was added to each well. The culture was continued in the incubator for 2 h. The absorbance at 450 nm was determined by an enzyme-labeled instrument.
6
Comprehensive Analysis of Fructus Puerariae
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A total of 13 batches of FP were collected from Zhejiang (S1), Guangdong (S2 and S3), Guangxi (S4, S5 and S6), Hebei (S8), Hunan (S9, S10, S11 and S12) and Sichuan (S13) provinces of China. Besides, the control herbs (S7) were purchased from the National Institutes for Food and Drug Control (Beijing, China). Reference standards (purity >98%) including rutin, daidzin, daidzein, and quercetin were obtained from the National Institutes for Food and Drug Control (Beijing, China). Glycitin, genistin, tectoridin, glycitein, genistein, tectorigenin, and biochanin A with the purities over 98% were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Nicotiflorin and ononin with the purities over 98% were bought from Chengdu Alfa Biotechnology Co., Ltd. (Chengdu, China). Meanwhile, formononetin and puerarin were bought from Chengdu Must Bio-Technology Co., Ltd.(Chengdu, China). HPLC grade acetonitrile (ACN) and methanol (MeOH) were obtained from Fisher Scientific (Fair Lawn, NJ, USA). Formic acid of HPLC grade was provided by Kermel Chemical Reagent Co., Ltd. (Tianjin, China) and pure water was obtained from Wahaha (Hangzhou, China). All the other reagents were of analytical grade.
7
Neuroprotective Effects of Natural Compounds
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Nutrients including L-tyrosine(CAS No.: 60-18-4), Vitamin B1(CAS No.: 67-03-8), Vitamin B2(CAS No.: 83-88-5), Vitamin B5(CAS No.: 137-08-6), Vitamin B6(CAS No.: 58-56-0), Vitamin B12(CAS No.: 68-19-9), Quercetin(CAS No.: 117-39-5), Resveratrol(CAS No.: 501-36-0), Tea Polyphenols(CAS No.: 84650-60-2), Genistein(CAS No.: 446-72-0), Baicalein(CAS No.: 491-67-8), Luteolin(CAS No.: 491-70-3), Proanthocyanidin(CAS No.: 4852-22-6) and Naringin(CAS No.: 10236-47-2) are all purchased from Yuanye Biotechnology (Shanghai, China); CCK8 kit and ROS kit are purchased from Beyotime Biotechnology (Shanghai, China).
Instruments includes Artificial climate box (ZRQ-400); Morris water maze (JLBehav-MWMG); Shuttle box (JLBehav-STG-4); Fluorescent dye 2′, 7′-Dichlorodihydrofluorescein diacetate (DCFH-DA, Sigma, USA).
Instruments includes Artificial climate box (ZRQ-400); Morris water maze (JLBehav-MWMG); Shuttle box (JLBehav-STG-4); Fluorescent dye 2′, 7′-Dichlorodihydrofluorescein diacetate (DCFH-DA, Sigma, USA).
8
UPLC-MS/MS Analysis of Isoflavonoids
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An Ultimate 3000 Ultra High Performance Liquid Chromatography (UPLC) system coupled with LTQ-Orbitrap velos pro mass spectrometer was used (Thermo-Fisher, Waltham, MA, USA), as well as an Acquity UPLC I-Class UPLC system coupled with Xevo TQ-S micro triple quadrupole mass spectrometer (Waters, Milford, MA, USA).
The reference compounds of formononetin, ononin, daidzein, daidzin, genistin, genistein, calycosin-7-O-β-d -glucoside, biochanin A, and chlorogenic acid (purity > 98% for all) were all purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Acetonitrile and formic acid were purchased from Fisher Scientific, San Jose, CA, USA Company (mass spec-grade). Deionized water was prepared by Milli-Q ultrapure water preparation system (Millipore Co., Ltd., Billerica, MA, USA). All other chemicals were purchased from Beijing Chemical Works (Beijing, China, analytical grade).
The reference compounds of formononetin, ononin, daidzein, daidzin, genistin, genistein, calycosin-7-O-β-
9
Fatty Acid and Sterol Profiling
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The sesame oil, rapeseed oil, and soybean oil were purchased from local markets (Wuhan, Hubei, China). Before being used for analysis, all samples were stored in the dark. The standard samples of fatty acid methyl ester (FAME), brassicasterol, ergosterol, campesterol, campestanol, stigmasterol, β-sitosterol, Δ5-avenasterol, cycloartanol, cycloartenol, 24-methylene-cycloartenol, and cholestanol were purchased from the Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Daidzin, daidzein, genistin, and genistein were purchased from the Shanghai Yuanye Biotechnology Co. (Shanghai, China). Methyl sterculate (C20H36O2, Purity ≥ 98 %) and methyl malvalate (C19H34O2, purity ≥ 95 %) were purchased from the Matreya Lipid and Biochemistry Technology Co., Ltd., USA. All solvents used were of analytical grade, and all standards were stored at −20 °C in darkness.
10
Quantitative Analysis of Isoflavones
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MEthanol, ethylene glycol and formic acid (analytically pure) were purchased from Xilong Science Co., Ltd. (Shantou, China). Acetonitrile (HPLC grade) was purchased from TEDIA Co., Ltd. (Fairfield, OH, USA). Ethanol (chromatographic pure) was bought from Xilong Science Co., Ltd. (Shantou, China). Puerarin, daidzin, daidzein and genistein (HPLC grade) were obtained from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Puerarin, daidzin, daidzein and genistein were prepared into 1000 mg/L standard stock solution with MEthanol. The stock solution was diluted to prepare a series of mixed standard working solutions as required.
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