White OptiPlate-96 (Perkin-Elmer, Norwalk, Connecticut, USA) microplates were coated with 10 μg/mL of nucleolin, RRM, RRM1, RRM2, RRM3, RRM4, GST, or OVA in carbonate buffer overnight at 4 °C. The coated plates were washed with PBS containing 0.05% Tween-20 and incubated with PBS containing 1% BSA for 1 h at 25 °C. After washing, 0.2 μM ODN1826, CpG K3, GpC K3, CpG K3(O), CpG D35, ODN1585, GpC D35, CRO or AS1411; or 2 μg/mL poly(I:C), all labeled with biotin, were incubated for 2 h at 25 °C, then with PE-labeled streptavidin (BioLegend) for 1 h at 25 °C. In the other experiment, the plates were incubated with 50 μg/mL PE-labeled MS-3 or PE-labeled isotype control antibody for 2 h at 25 °C. After washing the plates, fluorescence was measured using a multi-plate reader (DS Pharma Biomedical, Osaka, Japan).
White optiplate 96
Manufactured by PerkinElmer
Sourced in United States
The White OptiPlate-96 is a 96-well microplate designed for luminescence and fluorescence-based assays. It features a white opaque bottom that enhances signal detection and minimizes well-to-well crosstalk. The plate is made of high-quality polystyrene and is compatible with standard microplate readers.
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Lab products found in correlation
3 protocols using white optiplate 96
1
Nucleolin Binding Assay with ODNs
2
Quantification of Peptide-Streptavidin Binding
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Approximately 10 μg (equivalent to 28 pmol) of PE-labelled streptavidin was mixed with 2.8 nmol biotin-labelled DC-targeting peptide for 1 h at 25°C. The PE-labelled streptavidin and peptide complex was purified using an ultrafiltration tube. White OptiPlate-96 (Perkin-Elmer, Norwalk, CT, USA) was coated with 10 μg/mL recombinant mouse NRP-1 protein (R&D Systems) in 0.1 M sodium carbonate buffer (pH 9.6) overnight at 4°C. The coated plates were washed with PBS containing 0.05% Tween-20 and incubated with PBS containing 1% BSA for 1 h at 25°C. After washing, the plates were incubated with PE-labelled streptavidin and peptide complex for 2 h at 25°C. After washing the plates, fluorescence was measured using a multi-plate reader (DS Pharma Biomedical).
3
Nucleolin Binding Assay with Aptamers
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White OptiPlate-96 (Perkin-Elmer, Norwalk, Connecticut, USA) microplates were coated with 10 μg/mL recombinant nucleolin protein or 10 μg/mL OVA in carbonate buffer overnight at 4°C. The coated plates were washed with PBS containing 0.05% Tween-20 and incubated with PBS containing 1% BSA for 1 h at 25°C. After washing, 10 μg/mL of biotin-labelled Dp1×3 or biotin-labelled Dp2×3 was incubated for 1 h at 25°C, followed by PE-labelled streptavidin for 2 h at 25°C. In the other experiment, the plates were incubated with 0.5 μM biotin-labelled AS1411 in the presence or absence of 100 μg/mL Dp1×3 or Dp2×3, then with PE-labelled streptavidin for 2 h at 25°C. After washing the plates, fluorescence was measured using a multi-plate reader (DS Pharma Biomedical, Osaka, Japan).
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