Nod cb17 alhnrj prkdcscid rj mice

For knock-down xenograft experiments, MM1S cells were lentivirally infected with either shRNA constructs specifically targeting CD98hc and LAT1 or scrambled control shRNA. From both derived MM1S transduction-lines 0.6 × 10⁷ cells were suspended in PBS, mixed with Matrigel Basement Membrane Matrix (BD Bioscience) at a 1:1 ratio and injected subcutaneously into the opposite flanks of randomly selected female NOD.CB17/AlhnRj-Prkdc^scid/Rj mice 8–10 weeks of age (Janvier). For Anticalin xenograft experiments, 0.6 × 10⁷ wild-type MM1S cells were injected together with Matrigel s.c. into the right flank of female NOD SCID mice 8-10 weeks of age (Charles River). Mice were housed under SPF condition and animal experiments were conducted with permission from the District Government of Upper Bavaria (application no.: 55.2-2532.Vet_02-16-141 / 55.2-1-54-2532-216-15)

For CD98hc/LAT1 knockdown studies, female NOD.CB17/AlhnRj-Prkdc^scid/Rj mice 8–10 weeks of age (Janvier) were used. For Anticalin xenograft experiments female NOD SCID mice 8-10 weeks of age (Charles River) were used. Mice were housed under SPF condition and animal experiments were conducted with permission from the District Government of Upper Bavaria (application no.: 55.2-2532.Vet_02-16-141 / 55.2-1-54-2532-216-15).

Xenograft experiments were performed as previously described.²⁹ (link) In summary, 1.0 × 10⁷ MM cells were resuspended in serum-free medium, mixed with Matrigel Basement Membrane Matrix (Corning, Corning, NY) at a 1:1 ratio, and injected subcutaneously into the flanks of female NOD.CB17/AlhnRj-Prkdc^SCID/Rj mice 8 to 10 weeks of age (Janvier Labs, Le Genest-Saint-Isle, France). After tumor engraftment, mice were randomly assigned to be administered CFZ (2 mg/kg) IV, subasumstat (25 mg/kg) IV, a combination of both, or vehicle control twice per week. Treatment was performed for a total of 7 days. Tumor growth was monitored by caliper measurements. Mice were housed under specific pathogen-free conditions, and animal experiments were conducted in accordance with the local ethical guidelines and approved by the responsible regional authorities (District Government of Upper Bavaria; application no. ROB-55.2-2532.Vet_02-17-230).