Sunfire c18 rp

The following conditions were used for LC-HRESIMS analysis: capillary voltage 45 V, capillary temperature 260 °C, auxiliary gas flow rate 10–20 arbitrary units, sheath gas flow rate 40-50 arbitrary units, spray voltage 4.5 kV, and mass range 100–2000 amu (maximum resolution 30000). Gradient separation was achieved while using a SunFire C₁₈ RP analytical HPLC column (5µm, 4.6 × 150 mm, Waters) with a mobile phase of 0–100% MeOH over 30 min. at a flow rate of 1 mL/min. Multiple available databases (SciFinder: https://sso.cas.org/as/E9dPQ/resume/as/authorization.ping, MarinLit: http://pubs.rsc.org/marinlit/, Dictionary of Natural Products: http://dnp.chemnetbase.com/faces/chemical/ChemicalSearch.xhtml, PubChem: https://pubchem.ncbi.nlm.nih.gov/, Chemspider: http://www.chemspider.com/) were used for the dereplication and annotation of known compounds.

LC-HRESIMS spectrometric technique is composed of Thermo Instruments MS system (LTQ XL/LTQ Orbitrap Discovery) coupled to a Thermo Instruments HPLC system (Accela PDA detector, Accela PDA autosampler and Accela Pump). The following conditions were used: capillary voltage 45 V, capillary temperature 260°C, and auxiliary gas flow rate 10–20 arbitrary units, sheath gas flow rate 40–50 arbitrary units, spray voltage 4.5 kV, mass range 100–2000 amu (maximum resolution 30000). For LC/MS; Waters SunFire C₁₈ RP analytical HPLC column (5 μm, 4.6 × 150 mm) using a gradient of MeOH in H₂O containing 0.01% formic acid as eluent (0–100% over 30 min) at a flow rate 1 mL/min. (Marine Biodiscovery Centre, Chemistry Department, University of Aberdeen).

ESI-MS was performed for the sample from the 300 μM SA-treated fungal culture filtrate. Thermo Scientific MS system (XL/LTQ Orbitrap Discovery) coupled with a Thermo Scientific HPLC system (Accela PDA detector/autosampler/pump) was used. The following conditions were maintained in LC-MS analysis: capillary voltage 45 V, capillary temperature 260°C, auxiliary gas flow rate 10–20 arbitrary units, sheath gas flow rate 40–50 arbitrary units, spray voltage 4–5 kV and a mass range of 100–2000 amu (maximum resolution 30,000). Gradient separation was used on a Waters SunFire C18 RP analytical HPLC column (100Å, 5 μm, 4.6 mm × 150 mm) with a mobile phase of 0–100% MeOH over 30 min at a flow rate of 1 mL min^-1.