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P1075

Manufactured by Bio-Techne
Sourced in United Kingdom

The P1075 is a precision pipette from Bio-Techne. It is designed for accurate and reproducible liquid handling. The pipette offers adjustable volume ranges to suit various liquid transfer requirements.

Automatically generated - may contain errors

3 protocols using p1075

1

Cryo-EM Structural Analysis of SUR2 Proteins

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Purified SUR2B proteins were further supplemented with 3 mM fluorinated Fos-Choline-8 (FFC), 8 mM MgCl2, 8 mM ATP (Sigma) and 200 μM P1075 (Tocris Bioscience) for Mg-nucleotides + P1075 state; 8 mM MgCl2 and 8 mM ADP (Sigma) for Mg-nucleotides state; 8 mM MgCl2, 8 mM ADP and 200 μM Lev (MedChemExpress) for Mg-nucleotides + Lev state. Purified SUR2A proteins were supplemented with 3 mM FFC, 8 mM MgCl2, 8 mM ADP and 200 μM P1075 for Mg-nucleotides + P1075 state. Cryo-EM sample was loaded on to glow-discharged Quantifoil 0.6/1 gold grids and frozen as described previously19 (link).
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2

Reagent-Grade Chemical Synthesis Protocol

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Chemicals were of reagent grade and obtained from either Merck KG (Darmstadt, Germany) or Sigma-Aldrich (Saint Quentin Fallavier, France). TQ and P1075 were obtained from Tocris Bioscience (Bristol, UK).
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3

Potassium Channel Modulators in Cell Assays

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BTC/AM was obtained from BioNordika (Herlev, Denmark). Diazoxide, levcromakalim, nateglinide, P1075, PNU-37883A, repaglinide, Y-26763, and ZM226600 were purchased from Tocris (Bristol, UK). BMS-191095, gliclazide, and glimepiride were purchased from MedChemExpress (Sollentuna, Sweden). All other chemicals were obtained from Sigma-Aldrich. Test compounds were dissolved in anhydrous DMSO to generate 10 mM stock solutions and diluted in assay buffer before use. The final concentration of DMSO used was less than or equal to 1 % (v/v).
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