Lysozyme and lysostaphin

Manufactured by Merck Group

Sourced in China

Lysozyme and lysostaphin are enzymes that have the ability to break down the cell walls of certain bacteria. Lysozyme is found naturally in various body fluids, such as tears and saliva, and is known to be effective against a wide range of gram-positive and gram-negative bacteria. Lysostaphin, on the other hand, is primarily effective against Staphylococcus aureus, a common gram-positive bacterium. Both enzymes have potential applications in various fields, such as microbiology, biotechnology, and medicine.

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Lab products found in correlation

Bacterial genomic dna extraction kit, by Tiangen Biotech (1 mentions) La taq dna polymerase, by Takara Bio (1 mentions) Proteinase k, by Takara Bio (1 mentions) Xbai, by Takara Bio (1 mentions) Qubit dsdna hs assay kit, by Thermo Fisher Scientific (1 mentions) Genelute bacterial genomic dna kit protocol, by Merck Group (1 mentions) Ffpe dna kit protocol, by Qiagen (1 mentions)

Spelling variants of this product

Lysozyme (1136 citations) Lysostaphin (292 citations)

3 protocols using lysozyme and lysostaphin

Antibiotic Susceptibility Testing Protocol

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Antibiotic disks used for AST were purchased from Shanghai Kejia Drug Testing Equipment Co., Ltd; SmaI, XbaI, proteinase K, and La Taq DNA polymerase from Takara Co., Ltd; Lysozyme and lysostaphin from Sigma Aldrich, Beijing, China; Mueller-Hinton (MH) and Mannitol Salt agar from Guangdong Huankai Microbial Technology Co., Ltd; Agarose from Shanghai Shenggong Biological Co., Ltd; SeaKem Gold Agarose from Lonza Co., Ltd; and bacterial genomic DNA extraction kit from Tiangen Biochemical Technology Co., Ltd. The quality control strain used in this experiment was Staphylococcus haemolyticus ATCC25923, obtained from a culture bank.

Shoaib M., Xu J., Meng X., Wu Z., Hou X., He Z., Shang R., Zhang H, & Pu W. (2023). Molecular epidemiology and characterization of antimicrobial-resistant Staphylococcus haemolyticus strains isolated from dairy cattle milk in Northwest, China. Frontiers in Cellular and Infection Microbiology, 13, 1183390.

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Chronic foot infection model using S. aureus

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Following feeding, chronic foot infections were established. S. aureus (UAMS-1) was cultured overnight, washed, and diluted 1:10 in sterile saline. Mice were given 60mg/kg ketamine and 4mg/kg xylazine as well as a pre-operative dose of buprenorphine. Mice were then injected in the foot with 60μL of the diluted S. aureus. Immediately before and following the injection, the diameter of the footpad was measured using digital calpiers (Mitutoyo, Japan). All feet returned to the original size within 2 hours of injection. Feet were also measured at indicated time points. S. aureusdigest: UAMS-1 Δspa was digested at 37°C in the presence of lysozyme and lysostaphin (Sigma) for 3 hours in PBS. The digest was then washed prior to combination with alum (ThermoFisher) per manufacturer’s instructions. Following suspension with alum, 50μL of the S. aureus digest was immediately injected into the footpad of control or obese/T2D mice. Immunogenicity of the digest was confirmed prior to suspension in alum via an ELISA using serum from previously infected mice. Complete S. aureus killing in the digest was confirmed prior to alum suspension by plating on TSB agar plates overnight at 37°C.

Farnsworth C.W., Schott E.M., Benvie A., Kates S.L., Schwarz E.M., Gill S.R., Zuscik M.J, & Mooney R.A. (2018). Exacerbated S. aureus foot infections in obese/diabetic mice are associated with impaired germinal center reactions, Ig class-switching, and humoral immunity. Journal of immunology (Baltimore, Md. : 1950), 201(2), 560-572.

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DNA Purification from Protoblocks

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For purifying DNA from Protoblocks, unless specified, 10 μm × 15 μm sections aseptically collected sections were deparaffinized with ×2 xylene washes and processed following procedures specified in the QIAGEN FFPE DNA kit protocol (Qiagen Inc., Valencia, CA, USA). DNA was eluted in Tris-HCl (pH 8) and quantified with a Qubit^™ dsDNA HS Assay Kit (Invitrogen, USA). For non-fixed (NF) bacteria, bacterial cultures were grown to an OD₆₀₀ of 1. 2 ml aliquots were processed following procedures of the GenElute^™ Bacterial Genomic DNA Kit Protocol with lysozyme and lysostaphin (Sigma) and eluted in 50 µl of Tris-HCl (pH 8). In all cases, DNA was stored at −20°C until further analysis.

Flores Bueso Y., Walker S.P, & Tangney M. (2020). Characterization of FFPE-induced bacterial DNA damage and development of a repair method. Biology Methods & Protocols, 5(1), bpaa015.

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