Mouse anti ciap1

Manufactured by Santa Cruz Biotechnology

Sourced in Germany

Mouse anti-cIAP1 is a primary antibody that specifically targets the cellular Inhibitor of Apoptosis Protein 1 (cIAP1). cIAP1 is a member of the inhibitor of apoptosis (IAP) protein family and plays a role in regulating apoptosis and cell survival.

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Lab products found in correlation

2 protocols using mouse anti ciap1

Comprehensive Western Blot Analysis of Cell Death Regulators

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Western blot analysis was performed as described previously [29] (link), using the following antibodies: rabbit anti-MLKL, rabbit anti-RIPK3, rabbit anti-phospho MLKL, rabbit anti-phospho RIKP1, rabbit anti-phospho RIPK3, rabbit anti-TNFR1, rabbit anti-cIAP2 (all from Cell Signaling), mouse anti-RIPK1 (BD Bioscience), mouse anti-GAPDH (BioTrend), rabbit anti-TRAIL-R1 (Abcam), rabbit anti-TRAIL-R2 (Millipore), rabbit anti-FasR, mouse anti-cIAP1 (all from Santa Cruz), mouse anti-Caspase-8 (Enzo). For detection, secondary antibodies goat anti-mouse IgG and goat anti-rabbit IgG (Abcam) conjugated to horseradish peroxidase and enhanced chemiluminescence were used (Amersham Bioscience).

Koch A., Jeiler B., Roedig J., van Wijk S.J., Dolgikh N, & Fulda S. (2021). Smac mimetics and TRAIL cooperate to induce MLKL-dependent necroptosis in Burkitt's lymphoma cell lines. Neoplasia (New York, N.Y.), 23(5), 539-550.

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Immunoblotting of Cell Signaling Proteins

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Immunoblotting was performed using the following antibodies: mouse anti-cIAP1 (Santa Cruz, Heidelberg, Germany; 271418), rabbit anti-cIAP2 (Cell Signaling Technologies, Leiden, The Netherlands; 3130), rabbit anti-survivin (R&D; AF886), mouse anti-XIAP (BD, Franklin Lakes, NJ, USA; 610716), rabbit anti-IκBα (Cell Signaling Technologies; 9242), mouse anti-p-IκBα (Cell Signaling Technologies; 6249L), mouse anit-p65 (Santa Cruz; sc-8008), rabbit anti-p-p65 (Cell Signaling Technologies; 3033S), rabbit anti-NIK (Cell Signaling Technologies; 4994), mouse anti-p100 (Merck-Millipore, Darmstadt, Germany; 05-361), mouse anti-GAPDH (BioTrend, Köln, Germany; NB-29-00852), mouse anti-vinculin (Sigma/Merck; V9131), mouse anti-caspase-8 (Enzo; ADI-AAM-118-E), rabbit anti-caspase-9 (Cell Signaling Technologies; 9502S), rabbit anti-DR5 (Merck-Millipore; AB16942) and mouse anti-PARP-1 (Cell Signaling Technologies; 9546S). The following secondary horseradish peroxidase-conjugated antibodies were used: goat anti-mouse (Abcam, Berlin, Germany; ab6789) and goat anti-rabbit (Abcam; ab6721). The quantification of Western blot and the area determination of the region of interest were performed using ImageJ/FIJI [28 (link)]. Subsequent ratio calculations were performed using Microsoft Excel.

Särchen V., Reindl L.M., Wiedemann S., Shanmugalingam S., Bukur T., Becker J., Suchan M., Ullrich E, & Vogler M. (2023). Characterization of BV6-Induced Sensitization to the NK Cell Killing of Pediatric Rhabdomyosarcoma Spheroids. Cells, 12(6), 906.

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