Unicel dxc 600 synchron

Venous blood samples were collected on each testing day (pre, post₁, and post₂; between 8 and 10 a.m., and ~2 h after the athletes took a typical breakfast) from an antecubital arm vein of the right arm using a 20-gauge disposable Safety-Multifly^® needle (Sarstedt AG & Co, Nümbrecht, Germany) while the subject was in a supine position. Samples were collected into 7.5 mL serum gel tubes with clotting activator (Sarstedt AG & Co, Nümbrecht, Germany) and subsequently centrifuged at 3500 rpm for 15 min within 20 min after sampling. The resulting serum was separated from the other compounds, pipetted into micro tubes (Sarstedt AG & Co, Nümbrecht, Germany) and stored at -80°C. Later, routine techniques (UniCel^® DxC 600 Synchron^®, Beckmann Coulter GmbH, Krefeld, Germany) were used for analysis of the concentration of creatinkinase (CK), c-reactive protein (CRP), and urea. The diagnostic laboratory used in this study held current quality assurance certification (Referenzinstitut für Bioanalytik, Bonn, Germany).

Echocardiography was conducted using a Vivid E9 ultrasound system (GE Healthcare, Milwaukee, WI, USA). The main parameters used in the present study included FS, LVEF, LVMI, left ventricular end-diastolic diameter, interventricular septal thickness and left ventricular posterior wall thickness.
After overnight fasting, the peripheral venous blood of patients was collected using a vacutainer tube (without any anticoagulant) and an EDTA-coated tube between 8:00 am and 9:00 am. Serum samples were obtained by centrifuging the collected samples at 3500 rpm for 10 min at 4 °C, and plasma samples were obtained by centrifuging the collected samples at 2000 × g for 10 min at 4 °C. All blood samples were stored at −80 °C until further use.
HbA1c levels of whole blood samples were measured using high-performance liquid chromatography (Agilent 1200 device, Agilent Technologies, Waldbronn, Germany). Serum TC, TG, LDL-C, HDL-C, FBG, AST and ALT levels were determined using a biochemical autoanalyser (UniCel DxC 600 Synchron, Beckman Coulter, California, United States). NT-proBNP in the plasma was measured using an electrochemiluminescence immunoassay (ProBNP Elecsys®, Roche Diagnostics GmbH).

The principal variables of this study were albumin (independent variable) and CRP (dependent variable). The albumin concentration was measured using the DcX800 method which was as a bichromatic digital endpoint method. CRP was measured on the Beckman Coulter UniCel DxC 600 Synchron and the Beckman Coulter UniCel DxC 660i Synchron Access chemistry analyzers. In addition, the following covariates were included: age, alanine aminotransferase, blood urea nitrogen, creatinine, Gamma Glutamyl Transferase, body mass index, alkaline phosphatase, aspartate aminotransferase, cholesterol, creatine phosphokinase, globulin, lactate dehydrogenase, triglycerides, uric acid, total bilirubin, race/ethnicity, martial status, smoking, high blood pressure, gender. Details of albumin and CRP measurement process and other covariate acquisition process are available at www.cdc.gov/nchs/nhanes/.