Anti nis

Manufactured by Proteintech

Sourced in China

The Anti-NIS is a lab equipment product designed to detect the expression of the Sodium Iodide Symporter (NIS) protein. NIS is a membrane transport protein that plays a crucial role in iodide uptake in certain cell types, including thyroid cells. The Anti-NIS product can be used to measure and analyze the expression levels of the NIS protein in various experimental and research settings.

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Lab products found in correlation

Benzonase, by Merck Group (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions) β actin ac 15, by Merck Group (1 mentions) Anti myc tag 9b11, by Cell Signaling Technology (1 mentions) Anti ha y 11, by Santa Cruz Biotechnology (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Ebioscience foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (1 mentions) Nthy ori 3 1, by Thermo Fisher Scientific (1 mentions) Donkey anti rabbit igg h l, by Abcam (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions)

3 protocols using anti nis

TSH-Induced Thyroid Cell Signaling

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Y-PCCl3 cells were serum-starved for 24h, stimulated for 96h with 1 mU/mL TSH and then treated with TNF-α (10 ng/mL, 24h) or PMA (10 ng/mL, 24h). Protein extracts were prepared in Laemmli sample buffer (supplemented with 1 U/μl Benzonase, Sigma-Aldrich) and resolved, according to standard protocols, in 12% SDS-PAGE and transferred to PVDF membranes (Bio-Rad). The primary antibody rabbit polyclonal anti-NIS (Proteintech) was used in Western blot at 1:1000. Antibody mouse monoclonal anti-PCNA (Merck) was used at 1:10000. Detection was carried out using secondary peroxidase-conjugated anti-rabbit or anti-mouse IgG (Bio-Rad) antibodies followed by chemiluminescence.

Faria M., Domingues R., Paixão F., Bugalho M.J., Matos P, & Silva A.L. (2020). TNFα-mediated activation of NF-κB downregulates sodium-iodide symporter expression in thyroid cells. PLoS ONE, 15(2), e0228794.

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NIS Protein Expression Analysis

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Cells were harvested in RIPA (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% vol/vol Igepal CA-630, 6 mM sodium deoxycholate, 1 mM EDTA) with a protease inhibitor cocktail (Sigma). Western blotting was performed as described previously (31 (link)). Proteins (20 μg) were separated by SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) using 12% acrylamide gels. Primary antibodies used were rabbit polyclonal anti-NIS (1:1000; Proteintech, Rosemont, IL), mouse monoclonal anti-MYC-Tag 9B11 (1:1000; Cell Signaling Technology), rabbit monoclonal anti-HA Y-11 (1:1000; Santa Cruz), and mouse monoclonal β-actin AC-15 (1:10,000; Sigma).

Thompson R.J., Fletcher A., Brookes K., Nieto H., Alshahrani M.M., Mueller J.W., Fine N.H., Hodson D.J., Boelaert K., Read M.L., Smith V.E, & McCabe C.J. (2019). Dimerization of the Sodium/Iodide Symporter. Thyroid, 29(10), 1485-1498.

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Quantification of Thyroid Cell NIS Protein

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Nthy-ori 3-1 (a normal human thyroid follicular epithelial cell line) was ordered from Shanghai Royal Industrial Co. Ltd (Shanghai, China). Nthy-ori 3-1 cells were grown in RPMI-1640 (Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco), 100 IU/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells from different group were resuspended in PBS containing 2% FBS and 1 mL eBioscience™ Foxp3/Transcription Factor Staining Buffer Set (Invitrogen) for permeabilized conditions with 1 μL anti-NIS (Proteintech, Wuhan, China). After washing, cells were incubated with donkey anti-rabbit IgG H&L (Abcam). The fluorescence of 10⁷ cells per tube was assayed using the fluorescence-activated cell sorting (FACS) technique (BD Bio-Sciences, New York, NY, USA). Data were analyzed with FlowJo software (Tree Star, Ashland, Kentucky, USA).

Yang Q., Ke W., Pan F., Huang X., Liu J, & Zha B. (2023). Association between radioactive iodine uptake and neutropenia in untreated Graves’ disease. Endocrine Connections, 12(2), e220474.

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