Aztec analysis software

Manufactured by Oxford Instruments

Sourced in United Kingdom

Aztec is an analysis software developed by Oxford Instruments. It provides advanced data processing and visualization capabilities for a range of analytical techniques, including electron microscopy and X-ray analysis.

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Lab products found in correlation

Versa 3d dualbeam, by Thermo Fisher Scientific (2 mentions) Jsm it300lv, by JEOL (1 mentions) Evo 60, by Zeiss (1 mentions) X max 50 mm2 detector, by Oxford Instruments (1 mentions)

Close spelling variants of this product

Aztec® EDS analysis software AZtec software AZtec

5 protocols using aztec analysis software

Physicochemical Analysis of Chitosan Samples

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The elemental analysis of the Chi sample was performed in an organic elemental analyzer (INICUBE®, Elementar Americas Inc.) designed for simultaneous carbon, hydrogen, nitrogen, and sulfur analysis in solid and liquid samples. This analysis allows the detection of differences in the presence of sulfur atoms between Chi-S and Chi-C, which indicates sulfation degrees of Chi-C. Furthermore, Fourier transform infrared (FTIR) spectra of Chi-S and Chi-C were obtained with an ATR/FTIR interspec 200-X spectrometer. The FTIR spectra were recorded at 4 cm⁻¹ resolutions and 64 scans. Additionally, surface morphology and micro-analysis of Chi-S and Chi-C samples were observed by scanning electron microscopy (SEM model JSM-IT300LV, JEOL USA Inc.), coupled with energy dispersive X-ray analysis (EDS Aztec, Oxford Instruments); the elemental analysis was performed using a computer-controlled software (Aztec analysis software, Oxford Instruments).

Jiménez-Arriagada D., Hidalgo A.A., Neira V., Neira-Carrillo A, & Bucarey S.A. (2022). Low molecular weight sulfated chitosan efficiently reduces infection capacity of porcine circovirus type 2 (PCV2) in PK15 cells. Virology Journal, 19, 52.

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SEM Imaging of Microspheres and Scaffolds

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Microspheres and acellular scaffolds were imaged via a Versa 3D Dual Beam (FEI, Hillsboro, OR) scanning electron microscope (SEM) with a detector for energy dispersive spectroscopy (EDS). The BMP and TH11 microspheres were cryo-fractured using a sharp blade and the dispersion of BMP-2, TCP and HAp within the microspheres was further analyzed using EDS at an accelerating voltage of 10 kV. Pixel maps for atomic calcium, nitrogen and phosphorus were generated using Aztec analysis software (Oxford Instruments, Abingdon, United Kingdom). The PLGA (BLANK) microspheres were also imaged as a negative control to confirm the absence of calcium, nitrogen and phosphorous in the EDS maps.

Gupta V., Lyne D.V., Barragan M., Berkland C.J, & Detamore M.S. (2016). Microsphere-Based Scaffolds Encapsulating Tricalcium Phosphate And Hydroxyapatite For Bone Regeneration. Journal of materials science. Materials in medicine, 27(7), 121.

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Analyzing Microsphere Composition via SEM-EDS

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Microspheres and acellular scaffolds were imaged via a Versa 3D Dual Beam (FEI, Hillsboro, OR) scanning electron microscope with a detector for EDS. The microspheres were cryo-fractured using a sharp blade, and the dispersion of TGF-β₃, DCC, and CS within the microspheres was further analyzed using EDS at an accelerating voltage of 10 kV. Pixel maps for atomic nitrogen and sulfur were generated using Aztec analysis software (Oxford Instruments, Abingdon, UK). The PLGA (BLANK) microspheres were also imaged to confirm the absence of nitrogen and sulfur in the EDS maps (Supplementary Figure 1).

Gupta V., Tenny K.M., Barragan M., Berkland C.J, & Detamore M.S. (2016). Microsphere-based scaffolds encapsulating chondroitin sulfate or decellularized cartilage. Journal of biomaterials applications, 31(3), 328-343.

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Determining Plant Elemental Composition

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To determine the plant’s elemental composition, the powdered leaf and stem samples were subjected to EDX microanalyses. The powdered material was secured on aluminium stubs using carbon conductive tape and sputter-coated with gold using a Quorum Q150 GB Gold Coater (Laughton, East Sussex, UK). The samples were analyzed using an EDX detector on a Zeiss Ultra Plus FEG SEM (Smart SEM) (Oberkochen, Germany) at 5 kV, equipped with Aztec Analysis Software (Oxford Instruments, High Wycombe Buckinghamshire, UK).

Parusnath M., Naidoo Y., Singh M., Rihan H, & Dewir Y.H. (2023). Phytochemical Composition of Combretum molle (R. Br. ex G. Don.) Engl. & Diels Leaf and Stem Extracts. Plants, 12(8), 1702.

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SEM-EDS Analysis of Cross-Sections

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The cross-sections were analyzed by SEM-EDS in a Zeiss EVO 60 equipped with a lanthanum hexaboride filament operating at 20 keV. SEM images were collected in the backscattered electron (BSE) mode. EDS for elemental analysis was available with an X-Max 50 mm 2 detector and AZtec analysis software provided by Oxford Instruments. The detector was a silicon drift type, with a resolution of 127 eV FWHM and a detection limit of about 0.1 wt% percentage from 0.3 to 3 μm in depth. The software module uses a standardless ZAF quantification system. EDS elemental analysis was performed in a low pressure mode (100 Pa), without a conductive coating. The spot analyses were expressed both as atomic and weight percentages: the contribution of carbon and oxygen was excluded to obtain stoichiometric ratios and to assist in pigments' identification, as it was affected by the contribution of the embedding polyester resin.

Germinario G., Talarico F, & Torre M. (2022). Microanalyses and Spectroscopic Techniques for the Identification of Pigments and Pictorial Materials in Monet's Pink Water Lilies Painting. Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 28(1).

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