Five medicinal herbs was mixed as shown in Table 1 (total 5.0 kg; 30.0 g × 166.7) and extracted in a 10-fold mass of water at 100 °C for 2 h under pressure (1 kgf/cm2) using an electric extractor (COSMOS-660; Kyung Seo Machine Co., Incheon, Korea). The water extract was then filtered through a standard sieve (No. 270, 53 μm; Chung Gye Sang Gong Sa, Seoul, Korea). The solution was freezing-drying to give a powder using PVT100 freeze dryer (IlShinBioBase, Yangju, Korea). The yield of the GJT water extract was 9.75 % (487.5 g).
Pvt100 freeze dryer
Manufactured by IlShinBioBase
The PVT100 is a freeze dryer designed for laboratory applications. It is capable of removing water from samples through a freeze-drying process. The core function of the PVT100 is to preserve the physical and chemical properties of the samples by drying them under vacuum and low temperature conditions.
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3 protocols using pvt100 freeze dryer
1
Extraction of Medicinal Herb Blend
2
Standardized Preparation of Soshiho-Tang
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Herb components of SST were deposited in the K-herb Research Center, Korea Institute of Oriental Medicine (KIOM). Herbal decoction of SST was made by mixing herbal according to composition (Table 1
3
Standardized Preparation of SBS Decoction
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A standard stock solution of each reference compound was prepared at 1.0 mg/mL using methanol and stored at 4 °C until use. The allantoin stock solution was prepared at the same concentration using water. The SBS decoction was prepared at KIOM. The 12 medicinal herbs as described in Table 1 were mixed and extracted with distilled water at 100 °C for 2 h at 98 kPa pressure using an electric extractor (Cosmos-660; Kyungseo Machine Co., Incheon, Korea). The extracted solution was filtered using a standard sieve (No. 270, 53 μm; Chung Gye Sang Gong Sa, Seoul, Korea) and then the filtered solution was freeze-dried to give a powder using a PVT100 freeze dryer (IlShinBioBase, Yangju, Korea). The amount of lyophilized water extract produced was 705.0 g (yield: 14.1%). For quantitative analysis, 200 mg of freeze-dried SBS extract was dissolved in 20 mL of distilled water for 60 min at room temperature using a Branson 8510E-DTH ultrasonicator (Denbury, CT, USA). The extracted solution was filtered through a 0.2 μm membrane (Pall Life Sciences, Ann Arbor, MI, USA) before injection into the HPLC system.
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