Sc 166169

Five pairs of paired ovarian cancer and non-cancer ovarian epithelial tissues (RIPA Buffer Solaibao Institute of Biotechnology) were cleaved by the Western blotting assay, The lysate was produced by the centrifugation at 4℃ (12000 × g, 30min). The supernatant was cytoplasmic protein, which was then deposited into nucleoprotein. The cytoplasmic protein of the supernatant was introduced into the EP tube, and the protein concentration was measured with BCA protein determination kit. After 30 μg cytoplasmic protein was applied onto 10% SDS-PAGE, the protein underwent the transfer to polyvinylidene uoride membrane. After blocking (5% skimmed milk, 37°C 2h), the membrane underwent the incubation with PR antibody at 4 °C for 12 h (1:800 dilution; sc-166169; Santa Cruz Biotechnology), and then it was treated with horseradish peroxidase coupled secondary antibody (Goat anti-rabbit iggab6721, 1:5000, Abcam, UK) at 37 °C for 2 h. TBST solution was adopted to clean the lm, 6 times, 5min / time. Next, the gray value analysis of the obtained strips was conducted using gel image analysis software.