MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Paeoniflorin was obtained from the Huanyu Biotechnology Development Company (Beijing, China). A transwell chamber assay kit was purchased from BD Biosciences. An annexin apoptosis assay kit was purchased from Beyotime Biotechnology (Shanghai, China). Anti-TAZ, anti-β-catenin, and anti-tubulin antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).
Annexin apoptosis assay kit
Manufactured by Beyotime
Sourced in China
The Annexin apoptosis assay kit is a laboratory instrument designed to detect and quantify the level of apoptosis, a type of programmed cell death, in cell samples. The kit utilizes annexin V, a protein that binds to phosphatidylserine, a molecule that is exposed on the surface of apoptotic cells. The assay enables researchers to measure the degree of apoptosis in their experiments.
Automatically generated - may contain errors
Lab products found in correlation
Methyl thiazolyl tetrazolium (mtt), by Merck Group (2 mentions)
Anti tubulin antibody, by Cell Signaling Technology (1 mentions)
Anti taz, by Cell Signaling Technology (1 mentions)
Anti β catenin, by Cell Signaling Technology (1 mentions)
Matrigel, by BD (1 mentions)
Transwell chamber, by BD (1 mentions)
Goat anti mouse secondary antibody, by Cell Signaling Technology (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
2 protocols using annexin apoptosis assay kit
1
Evaluating Paeoniflorin's Anti-Cancer Effects
2
Skp2-Mediated Cell Cycle Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PF was purchased from the HuanYu Biotechnology Development Company. MTT was purchased from Sigma-Aldrich; Merck KGaA. The Annexin apoptosis assay kit was obtained from Beyotime Institute of Biotechnology. The Transwell chambers and Matrigel were purchased from BD Biosciences. Lipofectamine® 2,000 reagent was obtained from Invitrogen; Thermo Fisher Scientific, Inc. The anti-Skp2 (1:2,000; cat. no. 4358), anti-tubulin (1:5,000; cat. no. 2146), anti-β-actin (1:5,000; cat. no. 3700), anti-cyclin dependent kinase inhibitor 1A (p21; 1:1,000; cat. no. 2491) and anti-cyclin dependent kinase inhibitor 1C (p57; 1:1,000; cat. no. 2557) antibodies were obtained from Cell Signaling Technology, Inc. Goat anti-mouse secondary antibody (1:5,000; cat. no. A-11031) and goat anti-rabbit secondary antibody (1:5,000; cat. no. A-11034) were purchased from Thermo Fisher Scientific, Inc.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!