Dt ce

Manufactured by Glen Research

Sourced in Canada

The DT-CE is a laboratory equipment designed for DNA or RNA thermal cycling applications. It provides precise temperature control and cycling capabilities required for various molecular biology techniques such as PCR amplification. The DT-CE is a core functionality product without any further interpretation or extrapolation on its intended use.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using dt ce

Synthesis and Characterization of Modified DNA Oligonucleotides

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protected 2′-deoxyribonucleoside-3′-phosphoramidites (PAC-dA-CE, Ac-dC-CE, p-iPr-PAC-dG-CE, dT-CE, 8-oxo-dG-CE, 1,N⁶-etheno-dA-CE, 5-fluoro-dC-CE) Ac-dC-CPG ABI and p-iPr-PAC-dG-CPG ABI columns, and all other reagents necessary for automated DNA synthesis were purchased from Glen Research (Sterling, VA). 5′-O-(4,4′-dimethoxytrityl)-3′-O-(2-cyanoethyl)-N,N-diisopropyl-phosphoramidite of 6-chloropurine-2′-deoxyriboside was purchased from ChemGenes Corp. (Wilmington, MA). Synthetic DNA oligodeoxynucleotides were prepared by solid phase synthesis using an ABI 394 DNA synthesizer (Applied Biosystems, CA). DNA oligodeoxynucleotides containing 8-oxo-dG and thymine glycol were purchased from Integrated DNA Technologies (Coralville, IA) and Sigma Aldrich (St. Louis, MO), respectively. T4 polynucleotide kinase (T4-PNK) was obtained from New England Biolabs (Beverly, MA), while T4 DNA ligase was procured from Roche (Basel, Switzerland). γ-³²P ATP was purchased from Perkin-Elmer Life Sciences (Boston, MA). 40% 19:1 acrylamide/bis solution and micro bio-spin 6 columns were purchased from Bio-Rad (Hercules, CA). Illustra NAP-5 desalting columns and Sep-Pak C18 SPE cartridges were obtained from GE Healthcare (Pittsburg, PA) and Waters (Milford, MA), respectively. All other chemicals and solvents were purchased from Sigma-Aldrich (Milwaukee, WI) and used without further purification.

Wickramaratne S., Banda D., Ji S., Manlove A.H., Malayappan B., Nuñez N.N., Samson L., Campbell C., David S, & Tretyakova N. (2016). Base Excision Repair of N6-Deoxyadenosine Adducts of 1,3-Butadiene. Biochemistry, 55(43), 6070-6081.

+ Open protocol

+ Expand

Multifunctional Aptamer-Based Sensing Platform

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gold(iii) chloride hydrate, human serum type AB (male), silver nitrate, NaBH₄, thallium(i) chloride, cesium(i) chloride, rubidium(i) chloride, sodium chloride, magnesium(ii) chloride, iron(iii) nitrate, potassium(i) chloride, lead(ii) nitrate, silver(i) nitrate, lithium(i) chloride, nickel(ii) nitrate, streptavidin, PDDA (15%), zinc(ii) nitrate and mercury(ii) nitrate cadmium chloride(ii) were purchased from Sigma-Aldrich, Canada. Phosphoramidites dmf-dG-CE, dT-CE, dA-CE, Ac-dC-CE and biotin modifier were purchased from Glen Research, USA. ACN, TEAA, and anhydrous acetonitrile were purchased from BDH, VWR, Canada. Sample and absorption pad (CFSP223000) obtained from Millipore Co, Bedford, MA. Pullulan bought from Polysciences, Warrigton, PA. NC membrane was purchased from Sartorius Stedim Biotech, Germany and conjugate pad was purchased from Ahlstrom Munksjo, Finland. The aptamers used in this study were reported in the literature^7,37,38 and the aptamers were included in the ESI Table S1.†

Srinivasan S., Ranganathan V., McConnell E.M., Murari B.M, & DeRosa M.C. (2023). Aptamer-based colorimetric and lateral flow assay approaches for the detection of toxic metal ions, thallium(i) and lead(ii). RSC Advances, 13(29), 20040-20049.

+ Open protocol

+ Expand

Synthesis of Fluorescent DNA Amphiphiles

Check if the same lab product or an alternative is used in the 5 most similar protocols

The hydrophilic DNA component of the amphiphile was synthesized using standard phosphoramidite coupling on an Applied Biosciences 394 automated synthesizer with a 1000 Å CPG column (Glen Research) on a 1 µmol scale. Phosphoramidite monomers (bz-dA-CE #10-1000-10, Ac-dC-CE #10-1015-10, dmf-dG-CE #10-1029-10, dT-CE #10-1030-10, Fluorescein-dT-CE #10-1056-90 and Cyanine 5 Phosphoramidite #10-5915-95) were purchased from Glen Research and dissolved in dry solvent according to manufacturer instructions. Synthesis conditions include activator 4,5-Dicyanoimidazole (Glen Reasearch), 0.02 M iodine in THF/pyridine/water (Glen Research) as the oxidizer, 3% Trichloroacetic acid (Glen Research) as the deblocking solution, and capping mixtures THF/Pyridine/Ac2O (Glen Research) and 16% 1-Methylimidazole in THF (Glen Research). A 5'amino modifier (Glen Research) was coupled onto the end of sequences for subsequent conjugation with the polymer. Aliquots of each sequence (2-5 mg) were cleaved from the solid support and deprotected (concentrated ammonia for 24-36 hr) for analysis. The MMT protecting group of the amino modifier was left intact to act as a dragtag for HPLC purification. HPLC purified sequences were desalted using C18 resin (Ziptips, Millipore) and confirmed by MALDI-TOF using 2',4',6'-Trihydroxyacetophenone/ammonium citrate and 3-hydroxypicolinic acid as a matrix.

Nostrand E.L.V., Barnhill S.A., Shishkin A., Nelles D.A., Byeon E., Nguyen T.B., Wong Y.C.E., Gianneschi N.C., & Yeo G. (2020). Unbiased identification of nanoparticle cell uptake mechanism via a genome-wide CRISPR/Cas9 knockout screen.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!