Anti mouse igg hrp

D-(+)-Trehalose dehydrate (purity >99 %), toluidine blue, tert-Butyl hydroperoxide solution (TBHP), bafilomycin A1 (Baf), chloroquine (CQ) and type II collagenases were purchased from Sigma-Aldrich (St Louis, MO, USA). The primary antibody against p62, Tom20, BNIP3, PGAM5, Drp1, SOD2, PARP, cytochrome C, caspase 9, 8-0HdG and β-actin were from Abcam (Cambridge, UK), LC-3 antibody was from Sigma-Aldrich (St Louis, MO, USA). GRP78 and Calnexin antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA); Anti-CHOP, goat anti-rabbit, and anti-mouse IgG-HRP were from Bioworld (OH, USA) and antibodies against Atg3, Atg7, Atg12, Beclin1, p-AKT, AKT, p-mTOR, mTOR, p-p70S6K, p70S6K, p-AMPK, AMPK, p-ULK1, ULK1, ubquintin, caspase 12, Bcl-2, Bax and cleaved-caspase3 were from Cell Signaling (Danvers, MA, USA); Alexa Fluor488 labeled and Alexa Fluor594 labeled Goat Anti-Rabbit IgG (H+L) second antibody were from Jackson ImmunoResearch (West Grove, PA, USA). 4′,6-diamidino-2-phenylindole (DAPI) was from Beyotime (Shanghai, China).

Astilbin (purity > 98%) was purchased from BOC Sciences (New York, USA). Cell Counting Kit‐8 (CCK‐8) was purchased from Dojindo (Kumamoto, Japan). LPS, type II collagenase and dimethylsulphoxide (DMSO) were obtained from Sigma‐Aldrich (St Louis, MO, USA). The primary antibodies against TLR4, MyD88, TRAF6, Lamin B1 and GADPH were obtained from Abcam (Cambridge, UK); iNOS antibodies were obtained from Sigma‐Aldrich (St Louis, MO, USA); and MD2 antibody was purchased from eBioscience (eBioscience, SanDiego, CA). Antibodies against COX‐2, p65 and IκBα were purchased from Cell Signaling Technology (Danvers, MA, USA); anti‐IRAK1, goat anti‐rabbit and antimouse IgG‐HRP were from Bioworld (OH, USA). Recombinant human MD2 and TLR4 protein (rhMD2 and rhTLR4) were purchased from R&D Systems (Minneapolis, MN, USA). Biotin‐labelled LPS (Biotin‐LPS) was obtained from InvivoGen (San Diego, CA, USA). Alexa Fluor^®488 labelled and Alexa Fluor^®594 labelled goat anti‐rabbit IgG (H + L) the second antibody were purchased from Jackson ImmunoResearch (West Grove, PA, USA). 4′,6‐Diamidino‐2‐phenylindole (DAPI) was purchased from Beyotime (Shanghai, China), and cell culture reagents were obtained from Gibco (Grand Island, NY, USA).