Log In Sign up
The largest database of trusted experimental protocols

P50 binding elisa

Manufactured by Thermo Fisher Scientific
Visit Supplier

The P50 binding ELISA is a laboratory equipment product designed to detect and quantify the presence of P50 proteins in biological samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to measure the binding interaction between P50 and its specific antibodies.

Automatically generated - may contain errors

Lab products found in correlation

Tnf α, by Merck Group (2 mentions) Synergy 2 luminometer, by Agilent Technologies (2 mentions) Dual luciferase reporter assay, by Qiagen (2 mentions) Dual luciferase reporter assay system, by Promega (2 mentions)

Close spelling variants of this product

ELISAs

4 protocols using p50 binding elisa

1

NF-κB Activity Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Activity of NF-κB was determined by p50 binding ELISA (Thermo Scientific) performed according to manufacturer's protocol of whole cell lystates. Values were normalized to microgram protein, as measured by protein estimation (Thermo Scientific). Activation of NF-κB was stimulated by 20 ng/mL TNFα (Sigma).
Nomura A., Majumder K., Giri B., Dauer P., Dudeja V., Roy S., Banerjee S, & Saluja A.K. (2016). Inhibition of NF-kappa B pathway leads to deregulation of epithelial-mesenchymal transition and neural invasion in pancreatic cancer. Laboratory investigation; a journal of technical methods and pathology, 96(12), 1268-1278.
+ Open protocol
+ Expand
2

Quantifying NF-κB Activation Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
NF-κB activity was determined by both p50 binding ELISA (Thermo Scientific) and Dual-Luciferase reporter assay (Qiagen). Binding ELISA was performed according to the manufacturer’s protocol using whole cell lysates and values were normalized to μg protein as determined by protein estimation (Pierce). Dual-Luciferase reporter assay system (Promega) results were determined by Synergy2 luminometer (Biotek).
Nomura A., Gupta V.K., Dauer P., Sharma N.S., Dudeja V., Merchant N., Saluja A.K, & Banerjee S. (2017). NFkB-mediated Invasiveness in CD133+ Pancreatic TICs is Regulated by Autocrine and Paracrine Activation of IL-1 Signaling. Molecular cancer research : MCR, 16(1), 162-172.
+ Open protocol
+ Expand
3

NF-κB Activation Quantification Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
NF-κB activity was determined by both p50 binding ELISA (Thermo Scientific) and Dual-Luciferase reporter assay (Qiagen). Binding ELISA was performed according to the manufacturer's protocol using whole cell lysates and values were normalized to μg protein as determined by protein estimation (Pierce). Dual-Luciferase reporter assay system (Promega) results were determined by Synergy2 luminometer (Biotek).
Nomura A., Banerjee S., Chugh R., Dudeja V., Yamamoto M., Vickers S.M, & Saluja A.K. (2015). CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer. Oncotarget, 6(10), 8313-8322.
+ Open protocol
+ Expand
4

NF-κB Activity Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Activity of NF-κB was determined by p50 binding ELISA (Thermo Scientific) performed according to manufacturer's protocol of whole cell lystates. Values were normalized to microgram protein, as measured by protein estimation (Thermo Scientific). Activation of NF-κB was stimulated by 20 ng/mL TNFα (Sigma).
Nomura A., Majumder K., Giri B., Dauer P., Dudeja V., Roy S., Banerjee S, & Saluja A.K. (2016). Inhibition of NF-kappa B pathway leads to deregulation of epithelial-mesenchymal transition and neural invasion in pancreatic cancer. Laboratory investigation; a journal of technical methods and pathology, 96(12), 1268-1278.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!