Peroxidase conjugated avidin biotin complex

Manufactured by Vector Laboratories

Sourced in United States, Panama

Peroxidase-conjugated avidin-biotin complex is a reagent used in various immunohistochemical and biochemical applications. It consists of avidin, a protein that binds strongly to biotin, and horseradish peroxidase, an enzyme that can be used for signal detection. This complex is commonly used in techniques such as ELISA, Western blotting, and immunohistochemistry to amplify and detect target molecules.

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Lab products found in correlation

Biotinylated anti mouse igg, by Vector Laboratories (1 mentions) Rabbit anti rfp antisera, by Rockland Immunochemicals (1 mentions) Biotinylated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Dab peroxidase substrate kit, by Vector Laboratories (1 mentions)

Spelling variants of this product

Avidin-biotin complex (175 citations) Avidin-biotin-peroxidase (32 citations) Avidin-peroxidase complex (21 citations) Peroxidase-conjugated avidin (2 citations)

2 protocols using peroxidase conjugated avidin biotin complex

Immunostaining for Viral Proteins

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Expression of the viral proteins was detected by immunostaining as described previously [22 (link)]. Briefly, cells fixed with 50% acetone–50% methanol solution were serially incubated with the 6H4 anti-NS1 mouse monoclonal antibody [24 (link)], biotinylated anti-mouse IgG (Vector Laboratories, Burlingame, CA, USA), and peroxidase-conjugated avidin-biotin complex (Vector Laboratories). The cells with virus protein expression were visualized by incubation with the peroxidase substrate, 0.02% 3,3′-diaminobenzidine in 0.008% H₂O₂.

Ishikawa T., Narita K., Matsuyama K, & Masuda M. (2024). Dissemination of the Flavivirus Subgenomic Replicon Genome and Viral Proteins by Extracellular Vesicles. Viruses, 16(4), 524.

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Immunohistochemical Visualization of RFP-Positive Neurons

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To analyze the distribution of the red fluorescent protein (RFP)-positive neurons, brain tissue sections were treated with 1% H₂O₂ (Sigma-Aldrich) for 15 min to quench endogenous peroxidases, incubated in the blocking buffer for at least 2 hours, and then incubated overnight in rabbit-anti-RFP antisera (1:2,000; Rockland Immunochemicals, Gilbertsville, PA) at room temperature. Sections were then rinsed in PBS, incubated in biotinylated donkey anti-rabbit IgG (1:500; Jackson ImmunoResearch, West Grove, PA) for 2 hours, incubated with peroxidase-conjugated avidin-biotin complex (1:200; ABC, Vector Laboratories, Burlingame, CA) for 1 hour, followed by the addition of 3, 3’-diaminobenzidine tetrahydrochloride, H₂O₂ and NiSO₄ (DAB Peroxidase Substrate Kit, Vector Laboratories, Burlingame, CA) to visualize the reaction product.

Gerashchenko D., Schmidt M.A., Zielinski M.R., Moore M.E, & Wisor J.P. (2018). Sleep state dependence of optogenetically-evoked responses in neuronal nitric oxide synthase-positive cells of the cerebral cortex. Neuroscience, 379, 189-201.

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