Log In Sign up
The largest database of trusted experimental protocols

Abt 869

Manufactured by Selleck Chemicals
Visit Supplier

ABT-869 is a chemical compound used in laboratory research. It is a tyrosine kinase inhibitor that targets specific proteins involved in cellular processes. The core function of ABT-869 is to inhibit the activity of these proteins, which may be relevant for various areas of scientific investigation. Further details on the intended use of this product are not available.

Automatically generated - may contain errors

Lab products found in correlation

Cisplatin, by Selleck Chemicals (1 mentions) 5 fluorouracil 5 fu, by Selleck Chemicals (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Recombinant human epidermal growth factor, by R&D Systems (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Anti 4e bp1, by Cell Signaling Technology (1 mentions) Igf 1r, by Cell Signaling Technology (1 mentions) Bcl xl, by Cell Signaling Technology (1 mentions) Bcl 2, by Cell Signaling Technology (1 mentions) Vegfr2, by Cell Signaling Technology (1 mentions) Growth factor reduced matrigel, by BD (1 mentions) Pericentrin, by Abcam (1 mentions) Horseradish peroxidase hrp conjugated secondary antibody, by GE Healthcare (1 mentions) Lamin b1, by Thermo Fisher Scientific (1 mentions) Cyclin b1, by Cell Signaling Technology (1 mentions) Procaspase 3, by Cell Signaling Technology (1 mentions) Sns 032, by Selleck Chemicals (1 mentions) Phospho cdc2 y15, by Cell Signaling Technology (1 mentions) Alexa fluor 488 594 conjugated secondary antibodies, by Thermo Fisher Scientific (1 mentions) β actin, by Merck Group (1 mentions)

2 protocols using abt 869

1

Anticancer Agents and Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
ABT-869, 5-Fluorouracil (5-Fu) and cisplatin were purchased from Selleck Chemicals (Houston, TX). Recombinant human VEGF (VEGF165) was a gift from the Experimental Branch of the National Institutes of Health (Bethesda, MD). Recombinant human epidermal growth factor (EGF) and insulin-like growth factor (IGF) were purchased from R&D Systems (Minneapolis, MN). Growth factor-reduced Matrigel was obtained from BD Biosciences (San Diego, CA). The following antibodies were purchased from Cell Signaling Technology (Danvers, MA): VEGFR2, EGFR, IGF1R, AKT, mTOR, p70 S6K Kinase (S6K), eukaryotic initiation factor 4e (4EBP-1), poly (ADP-ribose) polymerase (PARP), Bcl-2, Bcl-xL, CyclinD1, phospho-specific anti-EGFR (Tyr1068), anti-IGF1R (Tyr1316), anti-VEGFR2 (Tyr1175), anti-AKT (Ser473), anti-mTOR (Ser2448), anti-S6K (Ser235/236) and anti-4EBP-1 (Thr37/46). Anti-CD31 antibody was obtained from Epitomics (Burlingame, CA).
Chen J., Guo J., Chen Z., Wang J., Liu M, & Pang X. (2016). Linifanib (ABT-869) Potentiates the Efficacy of Chemotherapeutic Agents through the Suppression of Receptor Tyrosine Kinase-Mediated AKT/mTOR Signaling Pathways in Gastric Cancer. Scientific Reports, 6, 29382.
+ Open protocol
+ Expand
2

Cdk1, Cdk5, and PCTAIRE1 Knockdown Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pre-designed small interfering RNA (siRNA) directed against human Cdk1 (s464), Cdk5 (s2825), PCTAIRE1 (1472, 1566, 1656), p27 (s2837), and negative scramble control (#1, #2), were purchased from Life Technologies. Kinase inhibitors (SNS-032 and ABT-869) were purchased from Selleckchem. Antibodies against PCTAIRE1 (mouse: G6.1 Santa cruz, or rabbit: HPA001366 Sigma), pro-caspase 3 (#9662, Cell Signaling), cleaved caspase 3 (#9661, Cell Signaling), cleaved PARP (mouse, Cell Signaling), p27 (mouse G173-524: BD, or rabbit C-19: Santa Cruz), phospho-p27 Ser10 (sc-12939, Santa Cruz), phospho-p27 Thr187 (37-9700, Invitrogen), phospho-p27 Thr198 (AF3994, R and D), Lamin-B1 (Invitrogen), phospho-Histone H3 (D2C8, Cell Signaling), Eg5 (611186, BD), Cdk1 (610037, BD), Cdk2 (610145, BD), Cyclin B1 (#4138, Cell Signaling), phospho-cdc2 (Y15) (#9111, Cell Signaling), pericentrin (ab4448, Abcam), alpha-tubulin (T5168, Sigma), HA (3F10, Roche), Myc (Roche), beta-actin (Sigma), horseradish-peroxidase (HRP)-conjugated secondary antibodies (GE Health Care), and Alexa Fluor 488/594-conjugated secondary antibodies (Life Technologies) were purchased from the indicated sources.
Yanagi T., Krajewska M., Matsuzawa S.I, & Reed J.C. (2014). PCTAIRE1 phosphorylates p27 and regulates mitosis in cancer cells. Cancer research, 74(20), 5795-5807.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!