Goat α rabbit fitc

Manufactured by Jackson ImmunoResearch

Goat α-rabbit-FITC is a secondary antibody conjugated with the fluorescent label FITC (Fluorescein Isothiocyanate). It is designed to bind to rabbit primary antibodies, allowing for their detection and visualization in various immunoassays and imaging techniques.

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Lab products found in correlation

Rat α ncad, by Developmental Studies Hybridoma Bank (1 mentions) Mouse α bruchpilot, by Developmental Studies Hybridoma Bank (1 mentions) Mouse α cd2, by Bio-Rad (1 mentions) Cy3 goat α mouse, by Jackson ImmunoResearch (1 mentions) Rat α elav, by Developmental Studies Hybridoma Bank (1 mentions) Rabbit α gfp, by Thermo Fisher Scientific (1 mentions) Fluoview fv1000, by Olympus (1 mentions) Goat α mouse cy2, by Jackson ImmunoResearch (1 mentions) Donkey anti rat cy5, by Jackson ImmunoResearch (1 mentions) Axioimager microscope, by Zeiss (1 mentions) Mouse α gfp, by BD (1 mentions) Donkey α mouse cy3, by Jackson ImmunoResearch (1 mentions) Dye conjugated secondary antibodies, by Jackson ImmunoResearch (1 mentions) Donkey α rabbit cy3, by Jackson ImmunoResearch (1 mentions)

2 protocols using goat α rabbit fitc

Immunohistochemical Staining of Fly Brains

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Staining of adult and pupal brains was performed as previously described [18 (link),24 (link)]. Primary antibodies were used in the following dilutions: rabbit α-GFP 1:1,000 (Invitrogen), rat α-Ncad 1:20, rat α-elav 1:100, mouse α-Bruchpilot 1:20 (Developmental Studies Hybridoma Bank), mouse α-CD2 1:1,000 (Serotec). The following secondary antibodies were used: goat α-rabbit-FITC 1:1000, goat α-mouse-Cy3 1:100, goat α-rat-Cy3 1:200, goat α-guinea pig-Cy3 1:500, goat α-rat-Cy5 1:200, goat α-rabbit-Cy5 1:500 (Jackson ImmunoResearch), Alexa 568 goat α-mouse IgG highly cross-adsorbed 1:300 (Invitrogen). Confocal images were taken using an Olympus Fluoview FV1000.

Hueston C.E., Olsen D., Li Q., Okuwa S., Peng B., Wu J, & Volkan P.C. (2016). Chromatin Modulatory Proteins and Olfactory Receptor Signaling in the Refinement and Maintenance of Fruitless Expression in Olfactory Receptor Neurons. PLoS Biology, 14(4), e1002443.

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Immunohistochemistry and In Situ Hybridization of Drosophila Imaginal Discs

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Imaginal disc immunohistochemistry and in situ hybridization were performed as previously described (Tanaka-Matakatsu and Du, 2008 (link)). Primary antibodies were used at the following dilutions: rabbit α-Ey 1:1000 (Halder et al., 1998 (link)) (gift from Uwe Walldorf), rabbit α-Da DAP7555 1:50 (Cronmiller and Cummings, 1993 (link)), mouse α-Eya 1: 40 (DSHB), rabbit α-Ato 1:2000 (Jarman et al., 1995 (link)), mouse α-β gal JIE7 Concentrated 1:500 (DSHB), mouse α-Glass 1:20 (DSHB), mouse α-Elav 1:50 (DSHB), mouse α-Myc 1:40 (9E10, DSHB), mouse α-GFP 1:500 (BD Bioscience). Dye conjugated secondary antibodies were from Jackson ImmunoResearch and used at 1:500 dilution: donkey α-mouse Cy3, donkey α-rabbit Cy3, goat α-mouse Cy2, goat α-rabbit-FITC, donkey anti-rat Cy5. DAPI was used at 1:100 (5ug/ml) for DNA staining. Images were taken using a Zeiss AxioImager microscope with an ApoTome.

Tanaka-Matakatsu M., Miller J., Borger D., Tang W.J, & Du W. (2014). Daughterless homodimer synergizes with Eyeless to induce Atonal expression and retinal neuron differentiation. Developmental biology, 392(2), 256-265.

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