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Recombinant human sphk1 and sphk2 proteins

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Recombinant human SPHK1 and SPHK2 proteins are laboratory reagents produced by BPS Biosciences. SPHK1 and SPHK2 are enzymes involved in the metabolism of sphingolipids. These recombinant proteins can be used for various in vitro research applications.

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Lab products found in correlation

Phosphorimager, by Bio-Rad (2 mentions) Silica gel g 60, by Cytiva (2 mentions)

2 protocols using recombinant human sphk1 and sphk2 proteins

1

Quantification of Sphingosine Kinase Activity

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Sphingosine kinase activity was determined as described previously28 (link). Recombinant human SPHK1 and SPHK2 proteins were obtained from BPS Bioscience. SPHK1 activity was determined in the presence of 0.25% Triton X-100, which inhibits SPHK228 (link). The labeled S1P was separated by TLC on Silica Gel G-60 (Whatman) with 1-butanol/ethanol/acetic acid/water (80:20:10:20, v/v) and visualized and quantified by phosphorimager (Bio-Rad).
Yamane D., McGivern D.R., Wauthier E., Yi M., Madden V.J., Welsch C., Antes I., Wen Y., Chugh P.E., McGee C.E., Widman D.G., Misumi I., Bandyopadhyay S., Kim S., Shimakami T., Oikawa T., Whitmire J.K., Heise M.T., Dittmer D.P., Kao C.C., Pitson S.M., Merrill AH J.r., Reid L.M, & Lemon S.M. (2014). Regulation of the hepatitis C virus RNA replicase by endogenous lipid peroxidation. Nature medicine, 20(8), 927-935.
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2

Quantification of Sphingosine Kinase Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sphingosine kinase activity was determined as described previously28 (link). Recombinant human SPHK1 and SPHK2 proteins were obtained from BPS Bioscience. SPHK1 activity was determined in the presence of 0.25% Triton X-100, which inhibits SPHK228 (link). The labeled S1P was separated by TLC on Silica Gel G-60 (Whatman) with 1-butanol/ethanol/acetic acid/water (80:20:10:20, v/v) and visualized and quantified by phosphorimager (Bio-Rad).
Yamane D., McGivern D.R., Wauthier E., Yi M., Madden V.J., Welsch C., Antes I., Wen Y., Chugh P.E., McGee C.E., Widman D.G., Misumi I., Bandyopadhyay S., Kim S., Shimakami T., Oikawa T., Whitmire J.K., Heise M.T., Dittmer D.P., Kao C.C., Pitson S.M., Merrill AH J.r., Reid L.M, & Lemon S.M. (2014). Regulation of the hepatitis C virus RNA replicase by endogenous lipid peroxidation. Nature medicine, 20(8), 927-935.
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