An overview of the antibodies used is presented in Table 1 and Table 2 . For DAB staining, we used a primary rabbit anti-RFP antibody (1:1000, Rockland Immunochemicals, Limerick, PA, USA) and a secondary donkey anti-rabbit antibody (1:200, Jackson, Cambridge, UK). For immunofluorescence, we used chicken anti-calbindin (1:500, Synaptic Systems, Goettingen, Germany), mouse anti-calbindinD-28 (1:500, Sigma-Aldrich), chick anti-GFP (1:200, Abcam, Cambridge, UK), goat anti-FoxP2 (1:500, Santa Cruz, Santa Cruz, CA, USA), guinea pig anti-vGluT2 (1:500, MilliPore, Amsterdam, The Netherlands), rabbit anti-RFP (1:1000, Rockland) and mouse anti-NeuN (1:1000, MilliPore) as primary antibodies. Cy5 anti-chicken (1:200, Jackson), Cy3 anti-mouse, FITC anti-chick, (1:200, MilliPore), Alexa488 anti-goat (1:200, Jackson), Cy5 anti-guinea pig (1:200, Jackson), Cy3 anti-rabbit (1:400, Jackson) and Alexa488 anti-Mouse (1:200, Jackson) antibodies were used as secondary antibodies.
For 3DISCO, we used chicken anti-calbindin (1:500, Synaptic Systems), goat anti-FoxP2 (1:500, Santa Cruz) and rabbit anti-RFP (1:000, Rockland) as primary antibodies. Cy5 anti-chicken (1:200, Jackson), Alexa488 anti-goat (1:200, Jackson) and Cy3 anti-rabbit (1:400, Jackson) antibodies were used as secondary antibodies.
For 3DISCO, we used chicken anti-calbindin (1:500, Synaptic Systems), goat anti-FoxP2 (1:500, Santa Cruz) and rabbit anti-RFP (1:000, Rockland) as primary antibodies. Cy5 anti-chicken (1:200, Jackson), Alexa488 anti-goat (1:200, Jackson) and Cy3 anti-rabbit (1:400, Jackson) antibodies were used as secondary antibodies.