Parental Ler-FRI, FLC::LUC line was described previously15 (link) and luciferase imaging was detected using a Nightowl imaging system (Berthold). Siliques valves were opened longitudinally to detect FLC::LUC signal from developing embryos. A complementing pELF6::ELF6::GUS::ELF6-3′UTR construction in elf6-1 mutant background was used to monitor ELF6 expression. FLC::GUS19 (link) was introgressed into the resetting mutant and β-glucuronidase activity was detected in Arabidopsis embryos. Siliques were longitudinally cut, fixed for 2 h at 20°C in 90% acetone and washed three times with 50 mM phosphate buffer (pH 7.0) before incubation at 37°C in reaction buffer for 24h (0.19 mM 5-bromo,4-chloro,3-indolyl-D-glucuronide, 10 mM EDTA, 0.1% Triton X-100, 0.1 mM KFeCN, 50 mM phosphate buffer pH 7.2). Embryos were observed after clearing in Hoyer’s medium using a microscope under bright-field Nomarski optics.
Nightowl imaging system
Manufactured by Berthold Technologies
The Nightowl imaging system is a specialized laboratory equipment designed for various imaging applications. It provides high-quality imaging capabilities for a range of scientific and research purposes. The core function of the Nightowl system is to capture and process images with precision and accuracy.
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Lab products found in correlation
3 protocols using nightowl imaging system
1
Monitoring Embryo Development and Gene Expression in Arabidopsis
2
Monitoring Embryo Development and Gene Expression in Arabidopsis
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Parental Ler-FRI, FLC::LUC line was described previously15 (link) and luciferase imaging was detected using a Nightowl imaging system (Berthold). Siliques valves were opened longitudinally to detect FLC::LUC signal from developing embryos. A complementing pELF6::ELF6::GUS::ELF6-3′UTR construction in elf6-1 mutant background was used to monitor ELF6 expression. FLC::GUS19 (link) was introgressed into the resetting mutant and β-glucuronidase activity was detected in Arabidopsis embryos. Siliques were longitudinally cut, fixed for 2 h at 20°C in 90% acetone and washed three times with 50 mM phosphate buffer (pH 7.0) before incubation at 37°C in reaction buffer for 24h (0.19 mM 5-bromo,4-chloro,3-indolyl-D-glucuronide, 10 mM EDTA, 0.1% Triton X-100, 0.1 mM KFeCN, 50 mM phosphate buffer pH 7.2). Embryos were observed after clearing in Hoyer’s medium using a microscope under bright-field Nomarski optics.
3
Fluorescence Imaging of BBBflamma Dyes in Mice
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Seven-week-old male BALB/c -nu/nu mice were obtained from Orientbio (Seongnam, Korea).
Fluorescence imaging was performed using the NightOWL imaging system (Berthold Technologies) equipped with a filter set (excitation at 475 nm and emission at 520 nm). BBBflammaTM 440 dye (molecular weight 500.61 g/mol) and BBBflammaTM 440-labeled SP600125 (molecular weight 803.94 g/mol) were kindly obtained from BioActs, Co. (Incheon, Korea). The drugs were intravenously and intraperitoneally injected (1 mg/kg) into mice. The mice were euthanized and the brain tissue was removed and scanned with an optical imager 24 h after drug injection. Image analysis was performed using the IndiGo program.
Fluorescence imaging was performed using the NightOWL imaging system (Berthold Technologies) equipped with a filter set (excitation at 475 nm and emission at 520 nm). BBBflammaTM 440 dye (molecular weight 500.61 g/mol) and BBBflammaTM 440-labeled SP600125 (molecular weight 803.94 g/mol) were kindly obtained from BioActs, Co. (Incheon, Korea). The drugs were intravenously and intraperitoneally injected (1 mg/kg) into mice. The mice were euthanized and the brain tissue was removed and scanned with an optical imager 24 h after drug injection. Image analysis was performed using the IndiGo program.
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