Triglyceride tr22421 reagents

Serum levels of ALT and AST were determined using the MaxDiscovery ALT (3460-08) and AST (5605-01) Assay Kits (Bioo-Scientific, Austin, TX). Plasma or tissue fatty acids, triglyceride and cholesterol were determined using the Infinity Cholesterol (TR13421) and Triglyceride (TR22421) Reagents (Thermo Scientific, Waltham, MA). Serum level of Lcn2 was measured by Lipocalin-2/ab199083 Mouse ELISA Kit (Abcam, Cambridge, MA). Serum level of Saa1 was measured by Saa1/ab157723 Mouse Elisa Kit (Abcam, Cambridge, MA). Serum level of FGF15 was measured by FGF15/LS-F11446 Mouse Elisa Kit (LifeSpan BioSciences, Inc, Seattle, WA). Serum levels of total adiponectin were determined using a commercial ELISA kit from BioVendor (Candler, NC). Serum levels of HMW forms of adiponectin were determined with Mouse Adiponectin Assay Kit from ALPCO # 47-ADPMS-E01 (Salem, NH). Concentrations of total bile acids in serum were determined using a 3α-hydroxysteroid dehydrogenase method (Diazyme). Analyses were performed with a SpectraMax i3x microplate reader (Molecular Devices, Sunnyvale, CA).

Plasma triglycerides and cholesterol concentrations were measured with an enzymatic assay (Konelab i 20XTi, Thermo Electron Corp., Waltham, MA) using commercially available reagents [Infinity cholesterol (TR13421) and triglyceride (TR22421) reagents, Thermo Fisher, Waltham, MA] and standards [Stabio cholesterol standard (SB1012030), Thermo Fisher, Waltham, MA; triglyceride standard (T7531STD), Pointe Scientific, Canton, MI]. Plasma HMW adiponectin was measured using a commercially available kit (EZHWAM-65, Millipore Sigma, Burlington, MA) previously validated for use in lean and obese horses (22 (link)). Average intra-assay variation for plasma adiponectin was 3.14%.