Alexa fluor 555 conjugated goat polyclonal anti rat

Manufactured by Thermo Fisher Scientific

Alexa Fluor 555–conjugated goat polyclonal anti-rat is a secondary antibody reagent. It is a goat-derived polyclonal antibody that is conjugated to the Alexa Fluor 555 fluorescent dye. This product is designed for use in immunofluorescence and other antibody-based detection applications.

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Lab products found in correlation

Streptavidin alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Rat anti mouse cd35 8c12, by BD (2 mentions) Biotin anti cd45.2 104, by BD (2 mentions) Streptavidin biotin blocking kit, by Vector Laboratories (1 mentions) A1 confocal microscope, by Nikon (1 mentions) Eclipse ti s microscope, by Nikon (1 mentions)

Spelling variants of this product

Alexa Fluor 555 (1070 citations) Alexa 555 (299 citations) Alexa Fluors (21 citations) Alexa Fluor 555 goat anti-rat (17 citations) Goat anti-rat Alexa 555 (12 citations) Anti-rat Alexa Fluor 555 (9 citations) Goat anti-rat 555 (5 citations) Alexa Fluor® 555-conjugated anti-rat (2 citations)

2 protocols using alexa fluor 555 conjugated goat polyclonal anti rat

Multicolor Immunofluorescence Staining

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These procedures were done as described (38 (link)). Streptavidin/Biotin Blocking Kit (Vector Labs) was used to block nonspecific binding. Tissue sections were stained in the following three steps: 1) with purified rat anti-mouse CD35 (8c12; BD Biosciences) plus biotin–anti-CD45.2 (104; BD Biosciences) or biotin-anti-PNA (B-1075; Vector Laboratories); 2) with Alexa Fluor 555–conjugated goat polyclonal anti-rat (Invitrogen) plus Alexa Fluor 488–streptavidin (Invitrogen); 3) with Alexa Fluor 647–conjugated rat antibody to mouse IgD (11-26C.2a; Biolegend). Mounted sections were imaged with a 20 × objective on a Nikon A1 confocal microscope.

Shi B., Geng J., Wang Y.H., Wei H., Walters B., Li W., Luo X., Stevens A., Pittman M., Li B., Thompson S.R, & Hu H. (2017). Foxp1 negatively regulates Tfh cell differentiation and germinal center responses by controlling cell migration and CTLA-4. Journal of immunology (Baltimore, Md. : 1950), 200(2), 586-594.

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Multicolor Immunofluorescence Staining Protocol

Cited 1 time

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These procedures were carried out as described previously (22 (link), 23 (link)). Antibodies used for staining: purified rat anti-mouse CD35 (8c12; BD Biosciences), biotin-anti-CD45.2 (104; BD Biosciences), Alexa Fluor 555-conjugated goat polyclonal anti-rat (Invitrogen), Alexa Fluor 488-Streptavidin (Invitrogen), Alexa Fluor 647-conjugated rat Ab to mouse IgD (11–26C.2a; Biolegend). Mounted sections were imaged with 10 × and 20 × objective on a Nikon Eclipse Ti-S microscope.

Geng J., Wei H., Shi B., Wang Y.H., Greer B.D., Pittman M., Smith E., Thomas P.G., Kutsch O, & Hu H. (2019). Bach2 negatively regulates Tfh cell differentiation and is critical for CD4+ T cell memory. Journal of immunology (Baltimore, Md. : 1950), 202(10), 2991-2998.

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