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Vector red ap chromogen

Manufactured by Vector Laboratories
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Vector Red AP chromogen is a substrate for alkaline phosphatase-based detection systems. It produces a bright red reaction product when cleaved by the enzyme, allowing for visualization of target analytes.

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Lab products found in correlation

Medical decloaking chamber, by Agilent Technologies (3 mentions) Target retrieval solution, by Agilent Technologies (3 mentions) Jc70 monoclonal antibody, by Roche (2 mentions) Immpress alkaline phosphatase kit, by Vector Laboratories (2 mentions) Ptgs2, by Cell Signaling Technology (1 mentions) Low ph target retrieval solution, by Agilent Technologies (1 mentions) Decloaking chamber, by Biocare Medical (1 mentions)

Close spelling variants of this product

Vector Red chromogen Vector Red

4 protocols using vector red ap chromogen

1

Immunostaining of Follicular Cells

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Follicles were fixed in 4% formaldehyde, embedded in paraffin, sectioned (7 μm), and processed for immunostaining. Briefly, heat-induced epitope retrieval was performed in a Biocare Medical Decloaking chamber utilizing Dako’s low pH Target Retrieval Solution. Primary antibody incubation was carried out at 4°C overnight for CD68, IL1B, and MMP9 (Supplementary Table 4). The antibody was detected using an appropriate Immpress alkaline phosphatase kit and Vector Red AP chromogen (Vector Laboratories) according to the manufacturer’s instructions. Slides were counterstained with hematoxylin. The negative control slides were prepared in an identical manner and processed without a primary antibody.
Tang J., Sawasdikosol S., Chang J.H, & Burakoff S.J. (1999). SLAP, a dimeric adapter protein, plays a functional role in T cell receptor signaling. Proceedings of the National Academy of Sciences of the United States of America, 96(17), 9775-9780.
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2

Immunohistochemical Analysis of Reproductive Markers

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Follicles were fixed in 4% formaldehyde, embedded in paraffin, sectioned (7
μm), and processed for immunostaining. Briefly, heat-induced epitope
retrieval was performed in a Decloaking Chamber (Biocare Medical) using a low pH
Target Retrieval Solution (Dako). Primary antibody incubation was carried out at
4°C overnight for PGR (prediluted; Dako), PTGS2 (1:200; Cell Signaling
Technology), and AREG (1:500; Sigma Chemical Company). The antibody was detected
using an appropriate ImmPRESS-AP alkaline phosphatase kit (Vector Laboratories)
and VECTOR Red AP chromogen (Vector Laboratories) according to
manufacturer’s instructions. Slides were counterstained with hematoxylin.
The negative control slides were prepared in an identical manner and processed
without primary antibody.
Choi Y., Wilson K., Hannon P.R., Rosewell K.L., Brännström M., Akin J.W., Curry TE J.r, & Jo M. (2017). Coordinated Regulation Among Progesterone, Prostaglandins, and EGF-Like Factors in Human Ovulatory Follicles. The Journal of Clinical Endocrinology and Metabolism, 102(6), 1971-1982.
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3

Immunostaining of ACE2 and PECAM1 in Tissue Sections

Cited 1 time
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The follicles were fixed in 4% formaldehyde, embedded in paraffin, and sectioned at 7 μm. Immunostaining was performed in the Markey Biospecimen Procurement and Translational Pathology Shared Resource Facility at the University of Kentucky, as previously described (28 (link)). Briefly, heat-induced epitope retrieval was performed in a Biocare Medical Decloaking chamber using Dako's low pH Target Retrieval Solution. Primary antibody incubation was performed at 4oC overnight for ACE2 (MilliporeSigma, HPA000288, 1:200 dilution) and for 2 hours at room temperature for platelet and endothelial cell adhesion molecule 1 (PECAM1) (Roche Diagnostics, Nederlands JC70 monoclonal antibody, predilute), respectively. Rabbit IgG was used in place of primary antibodies as a negative control. Antibody staining was detected with an appropriate Immpress alkaline phosphatase kit and Vector Red AP chromogen (Vector Laboratories, Burlingame, CA).
Choi Y., Jeon H., Brännström M., Akin J.W., Curry TE J.r, & Jo M. (2021). Ovulatory upregulation of angiotensin-converting enzyme 2, a receptor for SARS-CoV-2, in dominant follicles of the human ovary. Fertility and Sterility, 116(6), 1631-1640.
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4

Immunostaining of ACE2 and PECAM1 in Tissue

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Follicles were fixed in 4% formaldehyde, embedded in paraffin, and sectioned (7 μm). Immunostaining was conducted in the Markey Biospeciment Procurement and Translational Pathology Shared Resource Facility at the University of Kentucky as previously described (28 (link)). Briefly, heat-induced epitope retrieval was performed in a Biocare Medical Decloaking chamber utilizing Dako’s low pH Target Retrieval Solution. Primary antibody incubation was carried out at 4°C overnight for ACE2 (Sigma-Aldrich, HPA000288, 1:200 dilution) and for 2 h at room temperature for PECAM1 (Roche Diagnostics, JC70 monoclonal antibody, pre-dilute), respectively. Rabbit IgG was used in place of primary antibodies as a negative control. The antibody staining was detected using an appropriate Immpress alkaline phosphatase kit and Vector Red AP chromogen (Vector Laboratories).
Choi Y., Jeon H., Brännström M., Akin J.W., Curry TE J.r, & Jo M. (2021). Ovulatory upregulation of angiotensin-converting enzyme 2, a receptor for SARS-CoV-2, in dominant follicles of the human ovary. Fertility and Sterility, 116(6), 1631-1640.
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