Ektachem dt 60

After overnight fasting, baseline blood samples were obtained from each subject.
Glucose was measured by using a compact chemistry analyzer method (Ektachem DT-60; Eastman Kodak, Inc., Rochester, NY) with interassay CV of 2% (19 (link)).
Triglycerides and cholesterol were determined using a compact chemistry analyzer (Eastman Kodak) method, and an inter-assay coefficient of variation of 2.2% for triglycerides and 2% for cholesterol was found. Dextran-magnesium precipitation was used for high-density lipoprotein separation (19 (link)). Calculated low density lipoprotein levels was derived using the Friedewald formula. Albumin was determined using a calorimetric test (Vitros 950 ALB slides; J&J Health, Cone Systems, Piscataway, NJ, USA); the color complex formed was measured by reflectance spectrophotometry. The sensitivity of the assay has been shown to be 10 gL⁻¹; intra-assay CV were 1.3–1.5%. 25-hydroxyvitamin D (25[OH] D) level was measured as described elsewhere (20 (link)).

The absorbance of the samples was determined at 555 nm using an Ektachem DT 60 clinical chemistry analyzer (Eastman Kodak, Rochester, NY, USA) to analyze plasma lactate levels. Plasma MDA levels were analyzed using a BIOXYTECH LPO-586 kit (Oxis International, Beverly Hills, CA, USA). A 200-μL aliquot of plasma or standard was mixed with 640 μL of diluted N-methyl-2-phenylindole. Thereafter, 150 μL of concentrated hydrochloric acid was added, mixed, and incubated at 45 °C (60 min). After cooling, the absorbance values of the standards and samples were read at 586 nm using a spectrophotometer (HP 8452A; Hewlett-Packard, Palo Alto, CA, USA). Plasma SOD activity was analyzed using a tetrazolium-based kit (IBL, Hamburg, Germany). A 200 μL sample of the diluted radical detector and 10 μL plasma sample were added to prepared standard wells. Next, 20 μL of diluted xanthine oxidase was divided into the wells, mixed for several seconds, and incubated at 18–20 °C for 20 min. The absorbance was measured at 450 nm using a spectrophotometer (Tecan Sunrise; TECAN GmbH, Salzburg, Austria).