SDS-PAGE gels (13.5%) were run with a constant current of 30 mA and a voltage ceiling of 160V, and then transferred to a PVDF membrane for 1h at 100V. Membranes were blocked with 5% nonfat dry milk in TRIS-buffered saline with 0.1% Tween 20. Protein was detected with a primary mouse monoclonal anti-human activin A ab (clone 69403, R&D Systems) and a secondary HRP-conjugated goat anti-mouse ab (Life Technologies, Grand Island, NY, USA). Imaging was then performed with SuperSignal™ West Femto chemiluminescent substrate (Life Technologies) on an Alpha Innotech FluoroChem™ HD imager.
Anti human activin a ab clone 69403
Manufactured by R&D Systems
Sourced in United States
Anti-human activin A ab (clone 69403) is a monoclonal antibody that binds to activin A, a growth and differentiation factor that belongs to the transforming growth factor beta (TGF-β) superfamily. This antibody can be used for the detection and quantification of activin A in various biological samples.
Automatically generated - may contain errors
2 protocols using anti human activin a ab clone 69403
1
Activin A Protein Detection by Western Blot
2
Activin A Protein Detection by Western Blot
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