Approximately 100 µL of aqueous dispersion of drug-containing micelles (1.0 g/L) was transferred to a dialysis cell with MWCO of 2.0 kDa and then dialyzed against 3.4 mL of PBS buffer (pH 7.4) in the absence and presence of 10 U of esterase at 37°C or in the presence of serum and tumor homogenate at 37°C. The BUF concentration in the dialysate was quantified by high performance liquid chromatography (LC-20AD Series; Shimadzu Corporation, Kyoto, Japan) by measuring the absorption of BUF at 298 nm against a standard calibration curve.
Lc 20ad series
Manufactured by Shimadzu
Sourced in Japan
The LC-20AD Series is a liquid chromatography (LC) system developed by Shimadzu. It is designed to perform high-performance liquid chromatography (HPLC) analysis. The core function of the LC-20AD Series is to accurately and reliably deliver mobile phases for separation and detection of chemical compounds in a sample.
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Lab products found in correlation
2 protocols using lc 20ad series
1
Dialysis-Based Quantification of Drug Release
2
Aflatoxin B1 Degradation by Cunninghamella uredinicola
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A spore suspension of C. uredinicola (1 ml) was added to 250 ml conical flask with PD medium (50 ml) and proliferated for 34 h. Then, the cells (11.25 ml) were transferred to 250 ml conical flask with Czapek-Dox medium (75 ml) and fermented for 36 h. The above process took place at 28 °C and 180 rpm on a shaking incubator (Zhicheng ZWY-2102, Shanghai, China) with a rotational radius of 5 cm. After centrifugation (9408g, 10 min), the supernatant was collected in a new 50 ml tube. The pH of supernatant was adjusted to 7.0 by hydrochloric acid (0.5 mol/l).
Five µg AFB 1 was incubated with 1 ml culture supernatant at 37 °C for 24 h. Following the incubation, the residual AFB 1 and products were extracted by dichloromethane three times. Then, the dichloromethane was evaporated in vacuum drying oven (Jinghong D27-6090, Shanghai, China). Finally, the residual AFB 1 and products were dissolved in 1 ml methanol for HPLC (Shimadzu LC-20AD series, Japan) detection.
Five µg AFB 1 was incubated with 1 ml culture supernatant at 37 °C for 24 h. Following the incubation, the residual AFB 1 and products were extracted by dichloromethane three times. Then, the dichloromethane was evaporated in vacuum drying oven (Jinghong D27-6090, Shanghai, China). Finally, the residual AFB 1 and products were dissolved in 1 ml methanol for HPLC (Shimadzu LC-20AD series, Japan) detection.
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