To establish subcutaneous tumors with the A549 pair, 5-6 week old female athymic nude (nu/nu) mice (The Jackson Laboratory) were inoculated with 4×106 cells in 100μL of HBSS admixed with Matrigel 50% (v/v). All mice were housed and maintained in micro isolator cages under specific pathogen-free conditions and in accordance with the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) guidelines. All experimental studies were carried out under approval of the NIH Intramural Animal Care and Use Committee. Tumor volume was recorded; at the completion of the experiment, tumors were harvested and processed for immunohistochemical evaluation.
Athymic nude nu nu mice
Manufactured by Jackson ImmunoResearch
Sourced in United States, Montenegro
Athymic nude (nu/nu) mice are a strain of mice that lack a functional thymus gland, resulting in a deficiency of T cells. This mouse model is commonly used in research applications where the immune system response needs to be minimized, such as in the study of tumor growth and transplantation experiments.
Automatically generated - may contain errors
Lab products found in correlation
Matrigel, by Corning (2 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions)
Nod scid mice, by Jackson ImmunoResearch (1 mentions)
Digital caliper, by Avantor (1 mentions)
Matrigel basement membrane matrix, by BD (1 mentions)
Matrigel, by BD (1 mentions)
Matrigel matrix high concentration, by Corning (1 mentions)
Sonosite 180, by Fujifilm (1 mentions)
17 protocols using athymic nude nu nu mice
1
Subcutaneous Tumor Induction in Athymic Mice
2
Immunocompromised Athymic Nude Mice Care
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Male and female immunocompromised athymic nude (nu/nu) mice at 6 weeks of age were purchased from Jackson Laboratory and housed at Stanford’s Canary Center Animal Care Services. All animals were housed under standard conditions (12 hr:12 hr light:dark cycles temperature at 21.8 °C and humidity at 50%) and had free access to food and water. All studies were approved following review by Stanford’s Administrative Panel on Laboratory Animal Care, and all methods detailed in this manuscript were performed in accordance with these guidelines and regulations.
3
Athymic Nude and NOD/SCID Mouse Models
4
Subcutaneous Tumor Xenograft Model
5
Xenograft Tumor Establishment and Treatment
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Xenograft tumors were established in 6 weeks old female athymic nude (nu/nu) mice, obtained from Jackson Labs (Bar Harbor, ME) and maintained under pathogen limited conditions. All animal experiments were performed in accordance with the guidelines of Institutional Animal Welfare Committee. Animals were fed ad libitum with a standard diet. Tumor cells growing exponentially were harvested by brief incubation with 0.25% trypsin EDTA solution. The cells were washed and resuspended at a concentration of 3 × 107 cells/mL in PBS, which was then inoculated subcutaneously (s. c.) into the right flank of the mice. Tumor size was assessed using a digital caliper every other day after implantation and approximate tumor volume (mm3) was calculated as length × width2/2 (V = lw2/2). Treatment started when tumors were 150 mm3. Mice were randomized into three groups DOX, RBC-DOX, and control untreated (n = 6 per group). The DOX received 5 mg/kg IV doses twice a week for 4 weeks. The RBC-DOX group received an equivalent 5 mg/kg IV doses twice a week for 4 weeks. The control group received no treatment.
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Xenograft Model of HuR Knockouts
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Xenograft Tumor Growth Inhibition Study
8
In Vivo Tumor Xenograft Assay
9
Xenograft Tumor Growth Inhibition Study
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Two million cells in 200 μL Hank’s balanced salt solution were subcutaneously inoculated into the rear flanks of 5-week-old female athymic nude (nu/nu) mice (The Jackson laboratory, Bar Harbour, ME, USA). Once palpable, tumors were measured using Vernier calipers every other day. Tumor volumes were calculated using the formula: length×width×height×0.5236. When tumor volumes reached about 50 mm3, mice bearing each cell line were sorted into three groups to achieve equal distribution of tumor size in all the treatment groups. Group 1 intraperitoneally received 12 doses of the vehicle (1:1.8:0.4:6.8 mixture of DMSO, Kolliphor RH40, dextrose, and HEPES) while groups 2 and 3 received 12 doses of JG-231 (2 and 4 mg/kg body weight/dose, respectively). At the end of the experiment, animals were euthanized by CO2 asphyxiation. Investigators were not blinded. All animal studies were performed according to the protocols approved by the Institutional Animal Care and Use Committee at Medical College of Wisconsin, Milwaukee, WI, USA.
10
Xenograft Model of RIT1 and YAP
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Athymic nude (nu/nu) mice (4 to 6 weeks old) for the xenograft study were obtained from (Jackson Laboratory, Bar Harbor, ME, USA). SALE cells expressing RIT1 T76insTDLT , YAP 5SA , or RIT1 T76insTDLT + YAP 5SA were harvested by trypsinization, washed in PBS and resuspended at 10 6 cells/ml in PBS. Two hundred microliters (2 × 10 6 cells) were injected into each injection site, n=2 injection sites/mouse; 3 mice/condition for 6 replicates per cell line, in 4-6-week-old nu/nu mice. Cells were allowed to engraft for 1 week, then tumors were measu red ev ery 2-3 da ys using a digital caliper (VWR, Radnor, PA, USA). Tumor volume was calculated with the formula 0.5 × L × W 2 where L is the longest diameter and W is the diameter perpendicular to L. Tumors were monitored until the largest tumor reached 2 cm in diameter. All animal experiments were carried out in accordance with the Dana-Farber Cancer Institute Institutional Animal Care and Use Committee guidelines.
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