Pooled hlms xtreme 200

Seongsanamide A (>98%, Figure 1) was isolated from the fermentation broth extracts of Bacillus sp. KCTC 12796BP by as previously reported [7 (link)]. Acetaminophen, amodiaquine, bupropion, chlorzoxazone, dextromethorphan, trimipramine, uridine diphosphoglucuronic acid (UDPGA), nicotinamide adenine dinucleotide phosphate (NADP⁺), glucose-6-phosphate (G6P), and G6P dehydrogenase (G6PDH) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Coumarin, midazolam, omeprazole, and tolbutamide were purchased from Toronto Research Chemicals (Toronto, ON, Canada). Pooled HLMs (XTreme 200) were supplied by XenoTech (Lenexa, KS, USA). We purchased rP450 isoforms (rCYP1A2, rCYP2A6, rCYP2B6, rCYP2C8, rCYP2C9, rCYP2C19, rCYP2D6, rCYP2E1, rCYP2J2, rCYP3A4, and rCYP3A5) from Corning life sciences (Woburn, MA, USA). All solvents used in the analyses were LC-MS grade (Fisher Scientific Co., Pittsburgh, PA, USA).

Trans-resveratrol (tRVT, purity ≥ 99%), cis-resveratrol (cRVT, purity ≥ 98%), dehydronifedipine, diclofenac, 6-hydroxychlorzoxazone, 4-hydroxydiclofenac, 4-hydroxymephenytoin, S-mephenytoin, midazolam, mycophenolic acid (MPA), nifedipine, CDCA-24-acyl-β-glucuronide, 7-ethyl-10-hydroxy camptothecin (SN-38) glucuronide, MPA-β-d-glucuronide, and N-acetylserotonine (N-ASER)-β-d-glucuronide were obtained from Toronto Research Chemicals (Toronto, ON, Canada). Acetaminophen, amodiaquine, bupropion, chenodeoxycholic acid (CDCA), chlorzoxazone, dextromethorphan, dextrorphan, 6-hydroxybupropion, 7-hydroxycoumarin, N-ASER, N-desethylamodiaquine, naloxone, nifedipine, phenacetin, trifluoperazine (TFP), estrone-β-d-glucuronide, naloxone-β-d-glucuronide, and TFP-β-d-glucuronide were obtained from Sigma-Aldrich (St. Louis, MO, USA). SN-38 and 1′-hydroxymidazolam were provided by Santa Cruz Biotechnology (Dallas, TX, USA) and Cayman Chemicals (Ann Arbor, MI, USA), respectively. Pooled HLMs (XTreme 200) were purchased by XenoTech (Lenexa, KS, USA). rP450 (rCYP3A4, rCYP2E1, rCYP2C19, and rCYP1A2) were obtained from SPMED (Busan, Korea).