TCL cell lines (1 × 106 cells/mL) were incubated in 6-well plates for 24–48 h with romidepsin (2.5 nM) and lenalidomide (10 µM) either alone or in combination. After treatment, the cells were fixed and permeabilized using the BD Cytofix/Cytoperm Kit™ (BD Biosciences, San Jose, CA, USA) according to the manufacturer's instructions. Cells were incubated with FITC-conjugated mouse anti-human Bcl-2 monoclonal antibody (BD Biosciences, San Jose, CA, USA), or FITC-conjugated mouse IgG1 monoclonal isotype control antibody (BD Biosciences, San Jose, CA, USA), then analyzed by fow cytometry. The experiment was performed in triplicate.
Fitc conjugated mouse igg1 monoclonal isotype control antibody
Manufactured by BD
Sourced in United States
The FITC-conjugated mouse IgG1 monoclonal isotype control antibody is a laboratory reagent used in flow cytometry and other immunoassays. It serves as a control to help determine the specificity of target antibody binding and establish appropriate gating strategies.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fitc conjugated mouse igg1 monoclonal isotype control antibody
1
Bcl-2 Expression Analysis in TCL Cells
2
Bcl-2 Expression Analysis by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After treatment, the cells were fixed and permeabilized using the BD Cytofix/Cytoperm Kit™ (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions. Cells were incubated with FITC-conjugated mouse anti-human Bcl-2 monoclonal antibody (BD Biosciences, San Jose, CA, USA), or FITC-conjugated mouse IgG1 monoclonal isotype control antibody (BD Biosciences, San Jose, CA, USA), then analyzed by flow cytometry.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!