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4 protocols using ethylenediaminetetraacetic acid disodium salt edta 2na

1

Synthesis of Aqueous Electrolytes

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ZnSO4.7H2O (>99.0%), Li2SO4·H2O (>99.0%), and ethylene diamine tetraacetic acid disodium salt (EDTA-2Na, A.R.> 99.0%) were prepared from Sigma-Aldrich Chemical Co. All reagents were used directly without further purification. All aqueous electrolytes used deionized water as the solvent.
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2

Synthesis and Characterization of Graphene Oxide

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The following chemicals, graphite powder (Sigma–Aldrich, 10 mesh), sulfuric acid (Sigma–Aldrich, ACS reagent, 95.0–98.0%), hydrochloric acid (Sigma–Aldrich, ACS reagent, 37%), potassium permanganate (Fischer Scientific, ≥99%), hydrogen peroxide (Sigma–Aldrich, 30 wt% in H2O), sodium hydroxide (Sigma–Aldrich, ACS reagent, ≥97.0%), zinc acetate dehydrate (Sigma–Aldrich, ACS reagent, ≥98%) and phosphoric acid (Sigma–Aldrich, ACS reagent, ≥85 wt% in H2O). Scavengers p-benzoquinone (BQ), t-Butanol (TBA), ethylenediaminetetraacetic acid disodium salt (EDTA-2Na), and AgNO3 (all Sigma–Aldrich) were used as received. Zinc hydroxyacetate was prepared by procedure presented in our previous article28 (link).
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3

Antioxidant Compounds Characterization

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Rutin, quercetin, luteolin, apigenin, and luteolin-7-O-glucoside standards were obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). HPLC grade trifluoroacetic acid (TFA) and methanol were obtained from Aladdin Reagent Co., Ltd. (Shanghai, China). Ascrobic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ethylenediaminetetraacetic acid disodium salt (EDTA-2Na), sodium nitroprusside (SNP), potassium persulfate (K2S2O8), and trolox were obtained from Sigma-Aldrich (St. Louis, MO, USA). Other chemicals used (analytical grade) were bought from Kemio Chemical Reagent Co., Ltd. (Tianjin, China).
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4

Synthesis and Characterization of Magnetic Nanomaterials

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The organic pollutant OG
(7-hydroxy-8-[(E)-phenyldiazenyl] naphthalene-1,3-disulfonic
acid, C16H10N2Na2O7S2, azo dye) (Figure 1) and titanium dioxide (TiO2)
were purchased from Biochem, Germany, with purity >98%. To prepare
γ-Fe2O3, iron(II) chloride (FeCl2·4H2O, 99%) and iron(III) chloride (FeCl3, 97%) were purchased from Fluka and Sigma-Aldrich, Spain. Sodium
hydroxide (NaOH, 98%) and hydrogen chloride (HCl, 37%) were obtained
from Panreac, Germany. tert-Butanol (TBA), benzoquinone
(BQ), and ethylenediaminetetraacetic acid disodium salt (EDTA-2Na)
were purchased from Sigma-Aldrich. Hydrogen peroxide (H2O2, 30%) was purchased from SialChim Reagent Ltd., and
ethanol at 96.3% in analytical grade was obtained from VWR Chemicals.
Experimental solutions were prepared with doubly distilled water,
and all reagents were used without further purification.
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