Lef1 n 17

Manufactured by Santa Cruz Biotechnology

LEF1 (N-17) is a polyclonal antibody that specifically recognizes the N-terminus of the LEF1 (Lymphoid Enhancer Binding Factor 1) protein. LEF1 is a transcription factor that plays a crucial role in the Wnt signaling pathway, which is involved in various cellular processes such as cell fate determination, cell proliferation, and embryonic development.

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Lab products found in correlation

β catenin 610154, by BD (1 mentions) Axio observer inverted microscope, by Zeiss (1 mentions) Alexa fluor 594 labeled goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 labeled donkey anti goat igg, by Thermo Fisher Scientific (1 mentions) β catenin, by Thermo Fisher Scientific (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) Glass bottom plates, by MatTek (1 mentions)

2 protocols using lef1 n 17

Analyzing Wnt Pathway Components

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Western blotting, immunofluorescence staining and qRT-PCR were performed as previously reported (Ye et al., 2013 (link)). The primary antibodies used were LEF1 (N-17, Santa Cruz; 1:500), LEF1 (2286S, Cell Signaling Technology; 1:1000), TCF3 (M-20, Santa Cruz; 1:1000), TCF4 (H-125, Santa Cruz; 1:1000), TCF1 (2206S, Cell Signal Technology; 1:500), β-catenin (610154, BD Bioscience; 1:2000), and α-tubulin (32-2500, Invitrogen; 1:5000). The primers used are listed in Table S1.

Ye S., Zhang T., Tong C., Zhou X., He K., Ban Q., Liu D, & Ying Q.L. (2017). Depletion of Tcf3 and Lef1 maintains mouse embryonic stem cell self-renewal. Biology Open, 6(4), 511-517.

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Osteocyte Immunofluorescence Imaging Protocol

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WT and Sost KO osteocytes were grown on 12-well, collagen treated, glass bottom plates (MatTek Corporation). Cells were fixed in 4% PFA, washed in PBS, and blocked in 10% goat serum in PBS. The following primary antibodies were used: podoplanin (8.1.1, Santa Cruz Biotechnology, 1:50 dilution), Lef-1 (N-17, Santa Cruz Biotechnology, 1:50 dilution), β-catenin (Cell Signaling Technologies, 1:1000 dilution). After several PBS washes, secondary antibody was added. For podoplanin: Alexa Fluor 488-labeled goat anti-hamster IgG, 1:200 dilution; for Lef-1: Alexa Fluor 488-labeled donkey anti-goat IgG, 1:200 dilution; for β-catenin: AlexaFluor 594-labeled goat anti-rabbit IgG, 1:200 dilution (all from Life Technologies). Cells were washed with PBS, and counterstained with Vectashield Hard Set Mounting Medium containing 4’,6-diamidino-2-phenylindole (Vector Laboratories). Cells were photographed on an Axio Observer inverted microscope; fluorescence micrographs were taken using a Zeiss Super Resolution Elyra microscope system with Structured Illumination and a Plan-Apochromat 63X/1.4NA oil objective controlled by ZEN 2010 software.

Ryan Z.C., Craig T.A., Salisbury J.L., Carpio L.R., McGee-Lawrence M., Westendorf J.J, & Kumar R. (2014). Enhanced Prostacyclin Formation and Wnt Signaling in Sclerostin Deficient Osteocytes and Bone. Biochemical and biophysical research communications, 448(1), 83-88.

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